Two distinct adhesion mechanisms in embryonic neural retina cells. II. An immunological analysis.
Two distinct adhesion mechanisms in embryonic neural retina cells. II. An immunological analysis.
复制标题
胚胎神经视网膜细胞中有两种不同的粘附机制。
DOI：
10.1016/0012-1606(81)90352-3
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Steinberg,MS
中科院分区：
文献类型：
--
作者：
Thomas,WA;Steinberg,MS
针对神经视网膜细胞产生抗体，所述神经视网膜细胞通过在单独的EGTA（E细胞，Ca 2+非依赖性聚集）、在胰蛋白酶+Ca 2+（TC细胞，Ca 2+依赖性聚集）或在胰蛋白酶+ EGTA（TE细胞，非粘附性）中解离制备。抗E-细胞Fab选择性地抑制Ca 2+非依赖性聚集，抗TC-细胞Fab选择性地抑制Ca 2+依赖性聚集，而抗TE-细胞Fab两者都不抑制。来自第四种制剂的Fab也是针对E细胞培养的，它抑制Ca 2+非依赖性和Ca 2+依赖性聚集，但通过免疫吸附将其分离成两个部分，每种聚集模式各有一个特异性。在同时利用两种模式的细胞（LTC细胞）中，每种模式都被适当的Fab排他地抑制。这里提出的免疫学数据表明，在相同的细胞中存在两种不同的和功能上独立的粘附机制，每种机制负责两种聚集模式之一。通过不同程序制备的视网膜细胞的不同粘附特性可以通过这两种机制的存在、不存在或活性程度来解释，质量由组织解离中使用的胰蛋白酶和Ca 2+的浓度调节。
Antibodies were raised against neural retina cells prepared by dissociation in EGTA alone (E cells, Ca2+-independent aggregation), in trypsin + Ca2+(TC cells, Ca2+-dependent aggregation), or in trypsin + EGTA (TE cells, nonadhesive). Anti-E-cell Fab selectively inhibited Ca2+-independent aggregation, anti-TC-cell Fab selectively inhibited Ca2+-dependent aggregation, and anti-TE-cell Fab inhibited neither. Fab from a fourth preparation, also raised against E cells, inhibited both Ca2+-independent and Ca2+-dependent aggregation but was separated by immunoadsorption into two fractions, one specific for each mode of aggregation. In cells which utilize both modes simultaneously (LTC cells), each was inhibited exclusively by the appropriate Fab. The immunological data presented here demonstrate the existence in the same cells of two distinct and functionally independent adhesion mechanisms, each responsible for one of the two modes of aggregation. The differing adhesive properties of retinal cells prepared by different procedures are explained by the presence, absence, or degree of activity of these two mechanisms, qualities regulated by the concentrations of trypsin and Ca2+used in the tissue dissociation.