Biogenic synthesis and effect of silver nanoparticles (AgNPs) to combat catheter-related urinary tract infections
Biogenic synthesis and effect of silver nanoparticles (AgNPs) to combat catheter-related urinary tract infections
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DOI：
10.1016/j.bcab.2019.101037
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发表时间：
2019-03-01
影响因子：
4
通讯作者：
Selvin, Joseph
中科院分区：
文献类型：
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作者：
Divya, Meera;Kiran, George Seghal;Selvin, Joseph
银纳米颗粒（AgNPs）作为抗菌剂已经被考虑了几十年。与化学合成相比，生物合成AgNPs正成为一种重要而有效的方法。本研究主要以珊瑚相关菌为原料合成环保型AgNPs。共筛选了57株珊瑚菌分离株，选择MGL- D10作为AgNPs的合成菌株。基于16S rDNA序列的系统发育分析鉴定该分离物为Alcaligenes sp.。然后用紫外可见光谱、红外光谱和x射线衍射（XRD）对合成的AgNPs MGL- D10进行了表征。通过原子力显微镜（AFM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察合成的AgNPs的形貌和尺寸。显微分析表明，合成的AgNPs MGL- D10的尺寸为30-50 nm。合成的AgNPs MGL- D10对尿路感染（UTI）引起的临床分离株如芽孢杆菌（Bacillus sp.）、大肠杆菌（E. coll.）等具有抗菌活性。肺炎克雷伯菌、铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌。采用激光共聚焦扫描显微镜观察合成的AgNPs MGL- D10对金黄色葡萄球菌的抗菌膜作用。导管实验也证实了合成AgNPs MGL- D10的抗菌膜和抗菌作用。结果表明，将合成的AgNPs MGL- D10包覆在导管上可以有效抑制UTI致病菌的生长和生物膜的形成。本研究将为成功和生态友好的方法预防尿路感染引起的病原体和医院感染铺平道路。
Silver nanoparticles (AgNPs) have been considered as antimicrobial agents for decades. Rather than chemical synthesis, biosynthesis of AgNPs is emerging as a significant and effective method. This study mainly focused on synthesizing eco-friendly AgNPs from coral-associated bacteria. A total of 57 coral bacterial isolates were screened and the isolate MGL- D10 was selected for synthesizing AgNPs. The isolate MGL- D10 was identified as Alcaligenes sp. using 16S rDNA sequence based phylogenetic analysis. The synthesized AgNPs MGL- D10 was then characterized using UV-Vis spectroscopy, FTIR, and X-ray diffraction (XRD) analysis. Further, the morphology and the size of the synthesized AgNPs was observed through Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Microscopic analysis revealed the size of the synthesized AgNPs MGL- D10 was 30-50 nm. The synthesized AgNPs MGL- D10 showed antimicrobial activity against urinary tract infection (UTI) causing clinical isolates such as Bacillus sp., E. coll., K. pneumonia, P. aeruginosa, S. aureus and C. albicans. Antibiofilm effect of synthesized AgNPs MGL- D10 against S. aureus was assessed using confocal laser scanning microscopy. Catheter experiments also proved the antibiofilm and antimicrobial effect of synthesized AgNPs MGL- D10. The obtained results exhibit that the coating of synthesized AgNPs MGL- D10 on catheters effectively inhibited the growth and biofilm formation of UTI causing pathogens. The present study will pave a way for successful and eco-friendly methods of protection against urinary tract infection causing pathogens and nosocomial infections.