INVIVO SOLVENT-SUPPRESSED LOCALIZED HYDROGEN NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY - A WINDOW TO METABOLISM
INVIVO SOLVENT-SUPPRESSED LOCALIZED HYDROGEN NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY - A WINDOW TO METABOLISM
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DOI：
10.1073/pnas.82.7.2148
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ADAMS, WA
中科院分区：
文献类型：
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作者：
BOTTOMLEY, PA;EDELSTEIN, WA;ADAMS, WA
溶剂抑制NMR技术与脉冲磁场梯度和空间定位的表面线圈检测方法相结合。其结果是一种技术，使观察代谢物的氢（1H）NMR化学位移光谱从预选盘形体积的生物组织在体内。本地化的光谱记录从正常人的大脑和前臂，并从狗在采集周期为2秒，使用1.5-T成像/光谱系统。这比在体内获得类似的脑代谢物的最先进的31 P NMR光谱快几百倍。光谱实验之后是常规的表面线圈成像序列，以精确地定义所选的体积。脂质共振对光谱的污染是一个问题。
Solvent-suppression NMR techniques are combined with a pulsed magnetic field gradient and surface coil detection method of spatial localization. The result is a technique that enables observation of metabolites in the hydrogen (1H] NMR chemical-shift spectra from preselected disk-shaped volumes of biological tissue in vivo. Localized spectra are recorded from the normal human brain and forearm and from a dog in acquisition periods of 2 s using a 1.5-T imaging/spectroscopy system. This is several hundred-fold faster than acquiring similar state-of-the-art 31P NMR spectra of brain metabolites in vivo. Spectroscopy experiments are followed by conventional surface coil imaging sequences to precisely define the selected volume. Contamination of spectra by lipid resonances is a problem.