Murine macrophages kill the vegetative form of Bacillus anthracis
Murine macrophages kill the vegetative form of Bacillus anthracis
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DOI：
10.1128/iai.73.11.7495-7501.2005
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发表时间：
2005-11-01
影响因子：
3.1
通讯作者：
Cross, AS
中科院分区：
文献类型：
--
作者：
Kang, TJ;Fenton, MJ;Cross, AS
据报道，抗保护抗原抗体可以增强摄入的炭疽芽孢杆菌孢子的巨噬细胞杀死，但尚不清楚抗体介导的巨噬细胞杀伤机制是针对孢子本身还是从摄入和发芽的孢子中产生的植物形式的。为了解决这个问题，我们比较了鼠腹膜巨噬细胞杀死杀死发芽效率（GP）和发芽缺陷（Delta Gerh）34f2菌株孢子的杀戮。尽管巨噬细胞同样摄入了两个孢子，但只有GP Sterne在5 h时被杀死（0.37 log Kill）。用伽马干扰素（IFN-GAMMA）进行巨噬细胞的预处理或用从用炭疽疫苗免疫的受试者中分离出的免疫球蛋白G（IgG）的调子，分别增强了Sterne对0.49和0.73 log的杀死，但分别是IFN-GAMMA和IFN-GAMMA和IGGMA和IGGMA的组合没有任何一种治疗要好。在任何情况下，都没有杀死三角洲的孢子。为了检查外孢子保护孢子免受巨噬细胞的能力，我们比较了巨噬细胞介导的非封装（外孢子（+））和超声（外孢子（ - ））sterne 34f2孢子的杀死。超声波孢子的超声波孢子在5 h时被杀死（分别为0.98对0.37 log杀死）。 IFN-GAMMA的预处理增加了超声的孢子杀伤力增加到1.39日志。但是，使用IgG的调子化并不比没有对IFN-gamma进行治疗或预处理更好。我们得出的结论是，巨噬细胞似乎无法杀死炭疽芽孢杆菌的孢子形式，并且外孢子可能在保护孢子免受巨噬细胞的保护中发挥作用。
Anti-protective antigen antibody was reported to enhance macrophage killing of ingested Bacillus anthracis spores, but it was unclear whether the anti body-mediated macrophage killing mechanism was directed against the spore itself or the vegetative form emerging from the ingested and germinating spore. To address this question, we compared the killing of germination-proficient (gp) and germination-deficient (Delta gerH) Sterne 34F2 strain spores by murine peritoneal macrophages. While macrophages similarly ingested both spores, only gp Sterne was killed at 5 h (0.37 log kill). Pretreatment of macrophages with gamma interferon (IFN-gamma) or opsonization with immunoglobulin G (IgG) isolated from a subject immunized with an anthrax vaccine enhanced the killing of Sterne to 0.49 and 0.73 log, respectively, but the combination of IFN-gamma and IgG was no better than either treatment alone. Under no condition was there killing of Delta gerH spores. To examine the ability of the exosporium to protect spores from macrophages, we compared the macrophage-mediated killing of nonsonicated (exosporium(+)) and sonicated (exosporium(-)) Sterne 34F2 spores. More sonicated spores than nonsonicated spores were killed at 5 h (0.98 versus 0.37 log kill, respectively). Pretreatment with IFN-gamma increased the sonicated spore killing to 1.39 log. However, the opsonization with IgG was no better than no treatment or pretreatment with IFN-gamma. We conclude that macrophages appear unable to kill the spore form of B. anthracis and that the exosporium may play a role in the protection of spores from macrophages.