Polymer-Templated Gold Nanoparticles on Optical Fibers for Enhanced-Sensitivity Localized Surface Plasmon Resonance Biosensors
Polymer-Templated Gold Nanoparticles on Optical Fibers for Enhanced-Sensitivity Localized Surface Plasmon Resonance Biosensors
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DOI：
10.1021/acssensors.8b01372
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发表时间：
2019-03-01
期刊：
影响因子：
8.9
通讯作者：
Masson, Jean-Francois
中科院分区：
文献类型：
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作者：
Lu, Mengdi;Zhu, Hu;Masson, Jean-Francois
在光纤上的良好分散的金纳米颗粒（AuNPs）的密集阵列被示出为弥合局部表面等离子体共振（LSPR）和经典SPR传感之间的灵敏度和传感性能的差距。采用一种简单的自组装方法，在聚苯乙烯-b-4-乙烯基吡啶（PS-b-P4 VP）嵌段共聚物刷层上组装了直径为10 ~ 92 nm的金纳米粒子。与使用（3-氨丙基）三甲氧基硅烷（APTMS）和聚电解质的标准AuNP沉积方法相比，PS-b-P4 VP模板法的灵敏度被发现是3倍更好，颗粒之间的间隙较小和存在较少的AuNP聚集体的结果。因此，IgG的LSPR传感器的灵敏度是一个经典的SPR，也对光纤，约68%的棱镜为基础的波长询问SPR仪器。LSPR传感器的重现性和检测限与SPR传感器大致相同。使用PS-b-P4 VP嵌段共聚物制造方法的LSPR传感器的增强的性能为在更小且更具成本效益的平台中使用这些LSPR生物传感器铺平了道路。
Dense arrays of well-dispersed gold nanoparticles (AuNPs) on optical fibers are shown to bridge the gap in sensitivity and sensing performance between localized surface plasmon resonance (LSPR) and classical SPR sensing. A simple self-assembly method relying on a poly(styrene-b-4-vinylpyridine) (PS-b-P4VP) block copolymer brush layer was used to immobilize AuNPs of different diameters from 10 to 92 nm on optical fibers. In comparison with standard AuNP deposition methods using (3-aminopropyl)trimethoxysilane (APTMS) and polyelectrolytes, the sensitivity with the PS-b-P4VP templating method was found to be 3-fold better, a consequence of the smaller gap between particles and the presence of fewer AuNP aggregates. Hence, the sensitivity of the LSPR sensor for IgG was comparable to a classical SPR, also on optical fibers, and about 68% of that for a prism-based wavelength-interrogation SPR instrument. The reproducibility and the detection limit of the LSPR sensor were about the same as the SPR sensor. The enhanced performance of the LSPR sensors using the PS-b-P4VP block copolymer fabrication method paves the way for use of these LSPR biosensors in a smaller and more cost-effective platform.