Neuroprotective Effects of Formononetin Against NMDA-Induced Apoptosis in Cortical Neurons
Neuroprotective Effects of Formononetin Against NMDA-Induced Apoptosis in Cortical Neurons
复制标题
芒柄花素对 NMDA 诱导的皮质神经元细胞凋亡的神经保护作用
DOI：
10.1002/ptr.4928
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发表时间：
2013-12-01
影响因子：
7.2
通讯作者：
Zhao, Ming-gao
中科院分区：
文献类型：
--
作者：
Tian, Zhen;Liu, Shui-bing;Zhao, Ming-gao
Formononetin (FMNT) is an isoflavone found in many herbs including Trifolium pratense L., Spatholobus suberectus Dunn., and Astragalus mongholicus Bunge. The purpose of this study is to investigate pharmacological properties of FMNT on neurotoxicity induced by N-methyl-D-asparate (NMDA) in primary-cultured cortical neurons. The cell viability was significantly decreased after exposure to NMDA (200M) for 40min. Pretreatment of FMNT (10M) for 12h significantly attenuated the cell loss induced by NMDA exposure. Flow cytometry analysis revealed that treatment of FMNT attenuated the number of apoptotic cells, especially the early phase apoptotic cells, induced by NMDA exposure. Western blot analysis showed that FMNT regulated the expression of apoptosis-related proteins by increasing the levels of Bcl-2 and pro-caspase-3 and decreasing the levels of Bax and caspase-3. These findings demonstrate that FMNT is capable of protecting neurons from NMDA-evoked excitotoxic injury and has a potential perspective to the clinical treatment for neurodegenerative disorders in central nervous system. Copyright (c) 2013 John Wiley & Sons, Ltd.
