IMMUNOREACTIVITY OF A SURFACE WALL FRACTION PRODUCED BY SPHERULES OF COCCIDIOIDES-IMMITIS
IMMUNOREACTIVITY OF A SURFACE WALL FRACTION PRODUCED BY SPHERULES OF COCCIDIOIDES-IMMITIS
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DOI：
10.1128/iai.56.10.2695-2701.1988
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发表时间：
1988-10-01
影响因子：
3.1
通讯作者：
HEARN, VM
中科院分区：
文献类型：
--
作者：
COLE, GT;KIRKLAND, TN;HEARN, VM
免疫荧光和免疫扩散管沉淀试验表明，从球孢子虫液体培养物中分离得到的膜状球体外壁（SOW）与人抗球孢子虫抗体具有反应性。用非离子洗涤剂n -辛基- β从SOW中提取血清活性成分。-D-glucopyranoside (OG)。通过酶联免疫吸附试验，在25个球孢子菌病患者的血清样本中，SOW的og可溶性部分与免疫球蛋白G有反应。分离的母猪和母猪的og -可溶性部分也被证明能够诱导淋巴细胞形成。分别用二维免疫电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测og可溶性部分的抗原和蛋白质组成。根据球虫素-抗球虫素参比系统，从母猪中提取的抗原鉴定为抗原2和抗原CS。后者较早被分离出来，显示其分子质量（Mr）为19.9倍。在还原条件下SDS-PAGE检测到103。免疫印迹分析表明，同一电泳带与球孢子菌病患者血清具有反应性。另一种sod - page组分的og -可溶性部分，Mr为66倍。免疫印迹分析显示103与患者血清有反应。梭状芽胞杆菌的SOW是一个重要的免疫反应性壁成分储存库，以前没有报道过。
The membranous spherule outer wall (SOW) isolated from liquid cultures of Coccidioides immitis has been shown to elicit reactivity with human anti-Coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. The serologically reactive components were extracted from SOW with the nonionic detergent N-octyl-.beta.-D-glucopyranoside (OG). The OG-soluble fraction of SOW was shown to be reactive with immunoglobulin G in 25 serum samples from coccidioidomycosis patients by an enzyme-linked immunsorbent assay. The isolated SOW and OG-soluble fraction of SOW were also demonstrated to be capable of eliciting lymphocyte blastogenesis. The antigenic and protein compositions of the OG-soluble fraction were examined by two-dimensional immunoelectrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Two antigens which were extracted from SOW were identified as antigens 2 and CS on the basis of the coccidioidin-anticoccidioidin reference system. The latter was isolated earlier and shown to correspond to a molecular mass (Mr) of 19 .times. 103 by SDS-PAGE under reducing conditions. This same electrophoresis band was shown to be reactive with sera from coccidioidomycosis patients by immunoblot analysis. One other SDS-PAGE component of the OG-soluble fraction of SOW with an Mr of 66 .times. 103 was shown to be reactive with sera from patients by immunoblot analysis. The SOW of C. immitis represents an important reservoir of immunoreactive wall components which has not previously been reported.