Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection
Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection
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DOI：
10.1016/j.scienta.2011.01.021
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发表时间：
2011-04-11
影响因子：
4.3
通讯作者：
Guo, Wen-Wu
中科院分区：
文献类型：
--
作者：
Amar, Mohamed Hamdy;Biswas, Manosh Kumar;Guo, Wen-Wu
本研究旨在评价3种不同分子标记对24个柑橘及其近缘种遗传多样性的信息量和有效性。利用61个SSR标记、33个SRAP标记和24个CAPS-SNP标记对供试材料的多态性水平和鉴别能力进行了评价。在SSR引物中分别检测到596、656和135个多态性扩增产物。SRAP和CAPS-SNP标记的平均多态信息含量（PIC）分别为0.97、0.98和0.89。与CAPS-SNP标记相比，SSR和SRAP标记的多态性水平较高。SSR标记与SRAP标记的相关性最高（r = 0.930），与CAPS-SNP的相关性最低（r=0.833）。利用UPGMA法进行聚类分析，构建系统树。SSR数据构建的聚类图与常规聚类图的一致性最高。这些结果为今后有效利用这些分子标记进行柑橘及其近缘种的遗传分析奠定了基础。(C)2011 Elsevier B. V.保留所有权利。
The present study was to assess informativeness and efficiency of three different molecular markers for genetic diversity among 24 Citrus and its relative species. Sixty one SSR, 33 SRAP and 24 CAPS-SNP markers were used to evaluate the level of polymorphism and discriminating capacity. A total of 596,656 and 135 polymorphic amplicons were observed in SSR. SRAP and CAPS-SNP markers with average polymorphism information content (PIC) of 0.97, 0.98 and 0.89, respectively. High levels of polymorphism were recorded for SSR and SRAP compared with CAPS-SNP markers. The highest correlations (r = 0.930) were obtained between SSR and SRAP markers, whereas SSR and CAPS-SNP were poorly correlated (r=0.833). Cluster analysis was performed to construct dendrograms using UPGMA. And the dendrogram from SSR data was most congruent with the general dendrogram. These findings provide basis for future efficient use of these molecular markers in the genetic analysis of Citrus and its relatives. (C) 2011 Elsevier B.V. All rights reserved.