Post-embryonic functions of HSP90 in Tribolium castaneum include the regulation of compound eye development
Post-embryonic functions of HSP90 in Tribolium castaneum include the regulation of compound eye development
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DOI：
10.1007/s00427-011-0379-z
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发表时间：
2011-12-01
影响因子：
2.4
通讯作者：
Vilcinskas, Andreas
中科院分区：
文献类型：
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作者：
Knorr, Eileen;Vilcinskas, Andreas
热休克蛋白 90 (HSP90) 属于保守伴侣家族，在应激适应和发育中发挥多种作用，包括昆虫的精子发生、卵子发生和胚胎发生。在赤拟谷盗中，我们发现 HSP90 在幼虫发育过程中、预蛹、雌性蛹和成虫中短暂上调，表明其具有多种胚胎后作用。我们发现，通过 RNA 干扰沉默 HSP90 的表达在所有发育阶段都会在 10 天内致命。滴定实验表明，幼虫比蛹或甲虫更容易受到影响。有趣的是，最后龄幼虫中的 HSP90 沉默导致了异常的蛹表型，缺乏复眼并表现出预蛹特征，表明在预蛹阶段发育停滞。我们的结果表明，HSP90 的功能可以扩展到昆虫胚胎发生中已知的功能之外，包括在胚胎后发育中的作用，例如复眼发育的调节。
Heat shock protein 90 (HSP90) belongs to a family of conserved chaperons with multiple roles in stress adaptation and development, including spermatogenesis, oogenesis and embryogenesis in insects. In the red flour beetle, Tribolium castaneum, we found that HSP90 is transiently upregulated during larval development, in prepupae, in female pupae and in adults, suggesting multiple post-embryonic roles. We found that silencing HSP90 expression by RNA interference was lethal within 10 days at all developmental stages. Titration experiments revealed that larvae were more susceptible than pupae or beetles. Interestingly, HSP90 silencing in final instar larvae resulted in abnormal pupal phenotypes lacking compound eyes and exhibiting prepupal features, suggesting developmental arrest at the prepupal stage. Our results suggest that HSP90 functions can be expanded beyond the known ones in insect embryogenesis to include roles in post-embryonic development such as the regulation of compound eye development.