SINGLE-PARTICLE TRACKING - EFFECTS OF CORRALS
SINGLE-PARTICLE TRACKING - EFFECTS OF CORRALS
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DOI：
10.1016/s0006-3495(95)79911-8
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发表时间：
1995-08-01
影响因子：
3.4
通讯作者：
SAXTON, MJ
中科院分区：
文献类型：
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作者：
SAXTON, MJ
膜骨架的结构蛋白被认为在膜表面形成“皮层”，并且这些皮层可以限制膜蛋白的侧向扩散。单粒子跟踪和激光捕获的最新实验进展使得详细研究畜栏模型成为可能。技术来解释这些实验。首先，逃逸时间的扩散粒子在畜栏从Monte Carlo计算和各种畜栏的大小，形状和逃逸概率的解析解，并减少到一个共同的曲线。第二，考虑了跟踪实验中的目标识别问题。最简单的方法来识别corpus是通过视觉。如果壁是足够的不可渗透性，一个轨迹填充畜栏之前，扩散粒子逃逸。计算不同大小、形状和逃逸概率的皮质骨在逃逸前访问的不同部位的比例，并将其简化为一条共同的曲线。这个分数也是扩散物种在逃逸之前与畜栏中的另一物种反应的概率的度量。最后，研究了采样间隔对短程扩散系数测量的影响。
Structural proteins of the membrane skeleton are thought to form ''corrals'' at the membrane surface, and these corrals may restrict lateral diffusion of membrane proteins. Recent experimental developments in single-particle tracking and laser trapping make it possible to examine the corral model in detail. Techniques to interpret these experiments are presented. First, escape times for a diffusing particle in a corral are obtained from Monte Carlo calculations and analytical solutions for various corral sizes, shapes, and escape probabilities, and reduced to a common curve. Second, the identification of corrals in tracking experiments is considered. The simplest way to identify corrals is by sight. If the walls are impermeable enough, a trajectory fills the corral before the diffusing particle escapes. The fraction of distinct sites visited before escape is calculated for corrals of various sizes, shapes, and escape probabilities, and reduced to a common curve. This fraction is also a measure of the probability that the diffusing species will react with another species in the corral before escaping. Finally, the effect of the sampling interval on the measurement of the short-range diffusion coefficient is examined.