Sorting and lysis of single cells by BubbleJet technology
Sorting and lysis of single cells by BubbleJet technology
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DOI：
10.1016/j.snb.2012.04.005
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发表时间：
2012-06-20
影响因子：
8.4
通讯作者：
Zengerle, Roland
中科院分区：
文献类型：
--
作者：
Hoefemann, Henning;Wadle, Simon;Zengerle, Roland
我们证明了芯片集成的BubbleJet技术可以用于细胞分选以及单细胞裂解。在毫秒时间尺度上的蒸汽气泡是由在透明玻璃晶圆上制造的钛微加热器产生的。PDMS层粘合在玻璃晶圆的顶部并定义微流体通道。细胞分选是用小鼠成纤维细胞进行的，成纤维细胞具有流体动力学聚焦，运动速度约为500 μ m/s。对于细胞分选，气泡发生器放置在微流体通道外，但通过一个20 μ m的喷嘴连接到微流体通道。随机选择的成纤维细胞通过驱动从其原始流线向收集器出口偏转60 μ m。单细胞裂解是通过放置在细胞路径正下方的通道内的气泡发生器进行的。气泡的产生将细胞推向沟道顶部或沟道壁，由于巨大的剪切力，细胞以100%的效率被裂解。在实验中，分选和裂解的循环时间分别为5毫秒和20毫秒。(C) 2012 Elsevier B.V.版权所有
We demonstrate that chip-integrated BubbleJet technology can be used for cell sorting as well as for single-cell lysis. Vapor bubbles on the millisecond timescale are generated by titanium microheaters manufactured on transparent glass wafers. A PDMS layer is bonded on top of the glass wafer and defines microfluidic channels. Cell sorting is demonstrated with mouse fibroblasts which are hydrodynamically focused and travel at velocities of about 500 mu m/s. For cell sorting the bubble generators are placed outside the microfluidic channel but are connected to it via a 20 mu m nozzle. Randomly selected fibroblasts have been deflected laterally up to 60 mu m from their original streamline to a collector outlet by actuation. Single-cell lysis is performed by bubble generators placed inside a flow channel directly below the cells' path. Bubble generation pushes the cells towards the channel ceiling or the channel wall and cells are lysed due to the large shear force at an efficiency of 100%. Cycle times for sorting and lysis have experimentally been determined to be 5 ms and 20 ms, respectively. (C) 2012 Elsevier B.V. All rights reserved.