Paracrine effect of canine allogenic umbilical cord blood-derived mesenchymal stromal cells mixed with beta-tricalcium phosphate on bone regeneration in ectopic implantations
Paracrine effect of canine allogenic umbilical cord blood-derived mesenchymal stromal cells mixed with beta-tricalcium phosphate on bone regeneration in ectopic implantations
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DOI：
10.3109/14653249.2010.481665
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发表时间：
2010-01-01
期刊：
影响因子：
4.5
通讯作者：
Kweon, Oh-Kyeong
中科院分区：
文献类型：
--
作者：
Byeon, Ye-Eun;Ryu, Hak-Hyun;Kweon, Oh-Kyeong
背景目标。本研究旨在评价犬脐带血间充质干细胞（MSC）与β-磷酸三钙（β-TCP）混合后对异位种植骨再生的旁分泌作用。方法.将与cUCB MSC混合的β-TCP（UCB-MSC组）、细胞裂解物（细胞裂解物组）或对照（对照组）分别植入比格犬背部的皮下袋中。分别于植入后1、4、7、14、28、56、84天收集种植体。组织学结果和抗酒石酸酸性磷酸酶（TRACP）染色分析和碱性磷酸酶（ALP）和TRACP测定进行了评价。采用半定量逆转录-聚合酶链反应（RT-PCR）分析白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、环氧合酶-2（考克斯-2）、血管内皮生长因子（VEGF）和转化生长因子-β（TGF-β）的mRNA表达水平。采用酶联免疫吸附试验（ELISA）确认IL-6、考克斯-2、VEGF和TGF-β的蛋白表达水平。结果移植后7天，在所有组中均观察到TRACP阳性细胞。UCB-MSC组ALP和TRACP活性在移植后84天明显高于对照组（P
Background aims. The aim of this study was to evaluate the paracrine effects of canine umbilical cord blood (cUCB) mesenchymal stromal cells (MSC) mixed with beta-tricalcium phosphate (beta-TCP) on bone regeneration in ectopic implantation. Methods. beta-TCP mixed with cUCB MSC (UCB-MSC group), cell lysates (cell lysate group) or a control (control group) were respectively implanted in a subcutaneous pouches in the back of beagle dogs. The implants were harvested 1, 4, 7, 14, 28, 56, 84 days after implantation. Histological findings and stain analyzes of tartrate-resistant acid phosphatase (TRACP) and assays of alkaline phosphatase (ALP) and TRACP were evaluated. The mRNA expression levels of interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta) were analyzed using semi-quantitative reverse transcription - polymerase chain reactions (RT-PCR). An enzyme-linked immunosorbent assay (ELISA) was used to confirm the protein expression levels of IL-6, COX-2, VEGF and TGF-beta. Results. TRACP-positive cells were observed in all groups 7 days after implantation. ALP and TRACP activities in the UCB-MSC group 84 days after implantation were significantly higher than those of the control (P