Effect of short-term fasting and glucocorticoids on KLF15 expression and branched-chain amino acids metabolism in Chinese perch
Effect of short-term fasting and glucocorticoids on KLF15 expression and branched-chain amino acids metabolism in Chinese perch
复制标题
DOI：
10.1016/j.aqrep.2021.100617
复制
发表时间：
2021-03
影响因子：
3.7
通讯作者：
Xin Zhu;Jiang Hu;Jianshe Zhang;Jingjie Liu;Lingsheng Bao;Yaxiong Pan;Jia Hua Cheng;W. Chu
中科院分区：
文献类型：
--
作者：
Xin Zhu;Jiang Hu;Jianshe Zhang;Jingjie Liu;Lingsheng Bao;Yaxiong Pan;Jia Hua Cheng;W. Chu
骨骼肌代谢对营养变化有很强的适应性，就像在食物匮乏期间发生的那样。KRüppel-like factor15(KLF15)作为支链氨基酸(BCAAs)代谢的重要调节因子，在能量代谢中起着重要作用。但KLF15在鱼类骨骼肌适应和生理中的作用尚不清楚。在本研究中，我们获得了鳕鱼KLF15的完整基因，并研究了其在不同组织中的表达，以及短期禁食(3天或7天)和糖皮质激素(强的松龙)处理对其表达的影响。序列分析表明，ScKLF15与其他脊椎动物一样，在其C端具有高度保守的锌指C2H2超家族结构域。组织特异性分析表明，ScKLF15在快肌中高表达。在短期禁食期间，KLF15的表达在禁食3d后显著增加，禁食7d后显著下降，支链转氨酶2(BCAT2)的表达和丙氨酸氨基转移酶(ALT)的活性也显著降低。在强的松龙刺激下，KLF15和BCAT2的表达在注射后5d显著增加。此外，谷丙转氨酶(ALT)和天冬氨酸氨基转移酶(AST)的活性在注射后也呈现相同的趋势。因此，这项工作揭示了在面临食物剥夺和糖皮质激素胁迫时，支链氨基酸的分解代谢被增强，以维持鱼的生理动态平衡。更重要的是，本研究表明KLF15在河豚骨骼肌支链氨基酸代谢的适应性调节中起着重要作用。
Skeletal muscle metabolism is highly adaptive in response to nutritional changes, as occur during food deprivation. Krüppel-like factor 15 (KLF15), as a critical regulator of branched-chain amino acids (BCAAs) metabolism, which plays an important role in energy metabolism. But the role for KLF15 in adaptation and physiology of fish skeletal muscle remains unclear. In this study, we obtained the complete cDNA of Chinese perch (Siniperca chuatsi) KLF15 and investigated its expression in different tissues as well as its expression characteristics during short-term (3 or 7 days) fasting and glucocorticoid (prednisolone) treatment in Chinese perch. Sequence analysis indicated that ScKLF15 showed a highly conservative Zinc finger C2H2 superfamily domain at its C terminal with those of other vertebrates. Tissue specificity analysis showed that ScKLF15 was highly expressed in fast muscle. During the short-term fasting, the expression of KLF15 was significantly increased after 3 days fasting and decreased after 7 days fasting as well as the expression of branched-chain aminotransferase 2 (BCAT2) and the activity of alanine aminotransferase (ALT). Under the prednisolone stimulation, the expression of KLF15 and BCAT2 were significantly increased at 5 days post injection. Furthermore, the activity of ALT and aspartate aminotransferase (AST) showed the same tendency after the injection. Thus, this work revealed that the catabolism of BCAAs was enhanced to maintain the physiological homeostasis in the fish when faced with food deprivation and glucocorticoids stress. More importantly, the study suggested that KLF15 plays a role in adaptive regulation of BCAAs metabolism in skeletal muscle of Chinese perch.