Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
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DOI：
10.1309/ajcpgxk0fr4mhihb
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发表时间：
2010-11
影响因子：
3.5
通讯作者：
Skubitz AP
中科院分区：
文献类型：
--
作者：
Derycke MS;Pambuccian SE;Gilks CB;Kalloger SE;Ghidouche A;Lopez M;Bliss RL;Geller MA;Argenta PA;Harrington KM;Skubitz AP
卵巢癌的早期发现是困难的，因为缺乏具体和敏感的测试。此前，我们发现与正常卵巢相比，卵巢癌中Nectin 4的表达增加。逆转录酶-聚合酶链反应（RT-PCR）和定量RT-PCR验证了与正常卵巢细胞系和组织相比，卵巢癌中nectin 4信使RNA的过表达。与正常卵巢细胞系相比，卵巢癌细胞系和组织中nectin 4的蛋白水平升高，如通过Western免疫印迹、流式细胞术和组织微阵列载玻片的免疫组织化学染色所证明的。通过酶联免疫吸附测定，在许多患者血清样品中可检测到切割的连接蛋白4。在血清CA 125水平高的良性妇科疾病患者中，大多数病例未检测到nectin 4，这表明nectin 4可能作为一种潜在的生物标志物，有助于在CA 125组中区分良性妇科疾病和卵巢癌。
Early detection of ovarian cancer is difficult owing to the lack of specific and sensitive tests available. Previously, we found expression of nectin 4 to be increased in ovarian cancer compared with normal ovaries. Reverse transcriptase–polymerase chain reaction (RT-PCR) and quantitative RT-PCR validated the overexpression of nectin 4 messenger RNA in ovarian cancer compared with normal ovarian cell lines and tissues. Protein levels of nectin 4 were elevated in ovarian cancer cell lines and tissue compared with normal ovarian cell lines as demonstrated by Western immunoblotting, flow cytometry, and immunohistochemical staining of tissue microarray slides. Cleaved nectin 4 was detectable in a number of patient serum samples by enzyme-linked immunosorbent assay. In patients with benign gynecologic diseases with high serum CA125 levels, nectin 4 was not detected in the majority of cases, suggesting that nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125.