Characterizing the gut microbiota in females with infertility and preliminary results of a water-soluble dietary fiber intervention study
Characterizing the gut microbiota in females with infertility and preliminary results of a water-soluble dietary fiber intervention study
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DOI：
10.3164/jcbn.20-53
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发表时间：
2020-07-01
影响因子：
2.4
通讯作者：
Morimoto, Yoshiharu
中科院分区：
文献类型：
--
作者：
Komiya, Shinnosuke;Naito, Yuji;Morimoto, Yoshiharu
尽管辅助生殖技术取得了进步，但全世界约有8-12%愿意怀孕的人无法怀孕。生育能力取决于子宫内膜的接受状态、激素的适应以及免疫系统。局部和全身免疫受微生物群的影响很大。本研究的目的是比较女性患者和不孕不育对照组的肠道微生物群，并评估补充益生元部分水解瓜尔胶对辅助生殖技术治疗患者肠道生态失调和妊娠结局的影响。膳食纤维可以重建宿主肠道菌群，改变免疫功能；然而，关于膳食纤维治疗对辅助生殖技术成功的影响的临床数据缺乏。为了研究可育和不育雌性的肠道微生物群，我们对粪便样本进行了16S宏基因组分析。共招募18名可生育女性受试者和18名年龄匹配的不孕症患者，采集粪便样本，采用16S rRNA V3-V4测序分析肠道微生物组。未加权和加权主坐标分析表明，两组之间的微生物结构多样性存在差异。在不孕症患者中疣菌门的丰度较高。在属水平上，在不孕症患者中观察到窄养单胞菌、链球菌和玫瑰菌属的丰度下降，而未分类[Barnesiellaceae]和Phascolarctobacterium属的丰度增加。12名患者同意接受联合治疗，包括通过辅助生殖技术进行胚胎移植和口服补充部分水解瓜尔胶。联合治疗的妊娠成功率为58.3%(7/12)，失败率为41.7%（5/12）。治疗前妊娠的预测因素的特点是旁帕revotella和Blautia的丰度减少，双歧杆菌的丰度增加。治疗前妊娠的预测因素的特点是旁帕revotella和Blautia丰度的减少和双歧杆菌丰度的增加趋势。总之，本研究显示了可育组和不育组之间肠道菌群丰度的差异；此外，补充部分水解瓜尔胶有助于改善肠道生态失调和不孕女性的妊娠成功。
Despite the advances in assisted reproductive technology, approximately 8-12% of the individuals worldwide who are willing to conceive are unable to do so. Fertility depends on a receptive state of the endometrium and hormonal adaptations as well as the immune system. Local and systemic immunities are greatly influenced by the microbiota. The aim of the present study was to compare the gut microbiota in female patients with that in infertility with fertile control subjects and to evaluate the effect of prebiotic partially hydrolyzed guar gum supplementation on gut dysbiosis and the outcome of pregnancy in patients treated with assisted reproductive technology. Dietary fiber can reconstitute the host intestinal microbiota and modify the immune function; however, clinical data regarding the effect of dietary fiber treatment on the success of assisted reproductive technology is lacking. To investigate the gut microbiota in fertile and infertile females, we conducted 16S metagenomic analysis of fecal samples. In total 18 fertile female subjects and 18 patients with infertility matched by age were recruited, and fecal samples were obtained to analyze the gut microbiome using 16S rRNA V3-V4 sequencing. The unweighted and weighted principal coordinate analyses showed a trend indicating microbial structural differences in beta-diversity between these two groups. The abundance of the phylum Verrucomicrobia was higher in patients with infertility. At the genus level, a decrease in the abundance of the genera Stenotrophomonas, Streptococcus, and Roseburia and an increase in the abundance of the genera Unclassified [Barnesiellaceae] and Phascolarctobacterium was observed in patients with infertility. Twelve patients agreed to receive the combined therapy comprising embryo transfer by assisted reproductive technology and oral supplementation with partially hydrolyzed guar gum. The success of pregnancy by this combined therapy was 58.3% (7/12), and the failure was 41.7% (5/12). Predictive factors for pregnancy before treatment were characterized by a decrease in the abundance of Paraprevotella and Blautia and an increase in the abundance of Bifidobacterium. Predictive factors for pregnancy before treatment were characterized by a decrease in the abundance of Paraprevotella and Blautia and an increase tendency in the abundance of Bifidobacterium. In conclusion, the present study showed differences in the abundance of gut microbiota between fertile and infertile groups; moreover, partially hydrolyzed guar gum supplementation helped improve gut dysbiosis and the success of pregnancy in females with infertility.