Effects of retinoic acid on the concentrations of radioactive metabolites of retinol in tissues of rats maintained on a retinol-deficient diet.
Effects of retinoic acid on the concentrations of radioactive metabolites of retinol in tissues of rats maintained on a retinol-deficient diet.
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视黄酸对缺乏视黄醇饮食的大鼠组织中视黄醇放射性代谢物浓度的影响。
DOI：
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发表时间：
1991
影响因子：
2.1
通讯作者：
A. Lacroix
中科院分区：
文献类型：
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作者：
P. Bhat;A. Lacroix
研究了维生素A缺乏饮食的大鼠在组织中喂养视黄酸2天和6天对标记视黄醇代谢的影响。用高效液相色谱法分析了视黄醇的代谢产物。喂食视黄酸2天可显著降低血液中视黄醇和视黄酯的水平，而不影响肝脏中维生素A的含量。在小肠和睾丸中，维甲酸的含量显著降低。在饲料中添加视黄酸6天，除了降低血液中的视黄醇和视黄酯值外，还导致肝脏中的视黄酯值增加。维甲酸饲养大鼠肝脏中的视黄酯的积累伴随着标记的维甲酸的情况下。发现肾脏组织中含有最高水平的标记维甲酸，视黄醇和视黄酯;在实验期间，饮食维甲酸并没有改变这些维甲酸在肾脏中的浓度。由于肾脏保留更多的维生素A时，肝脏维生素A是低的，也饮食中的视黄酸不影响放射性视黄酸在肾脏中的浓度，这表明，肾脏可能发挥了重要作用，在生产视黄酸从视黄醇在体内。
The effects of feeding retinoic acid for 2 and 6 days on the metabolism of labeled retinol in tissues of rats maintained on a vitamin A deficient diet was studied. The metabolites of retinol were analyzed by high performance liquid chromatography. Feeding retinoic acid for 2 days significantly reduced the blood retinol and retinyl ester levels without affecting the vitamin A content of the liver. In intestine and testis the content of labeled retinoic acid was decreased significantly by dietary retinoic acid. Addition of retinoic acid to the diet for 6 days resulted, in addition to decreased blood retinol and retinyl ester values, in an increase in the retinyl ester values in the liver. The accumulation of retinyl ester in the retinoic acid fed rat liver was accompanied by an absence of labeled retinoic acid. Kidney tissue was found to contain the highest levels of labeled retinoic acid, retinol, and retinyl esters; dietary retinoic acid did not alter the concentrations of these retinoids in the kidney during the experimental period. Since kidney retained more vitamin A when the liver vitamin A was low and also dietary retinoic acid did not affect the concentrations of radioactive retinoic acid in the kidney, it is suggested that the kidney may play a major role in the production of retinoic acid from retinol in the body.