Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells.
Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells.
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DOI：
10.1371/journal.pone.0138704
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cheeran MC
中科院分区：
文献类型：
--
作者：
Krishna VD;Roach E;Zaidman NA;Panoskaltsis-Mortari A;Rotschafer JH;O'Grady SM;Cheeran MC
干扰素 (IFN) 已被证明可以抑制甲型流感病毒 (IAV) 复制，并在控制病毒感染中发挥重要作用。在这里，我们研究了原代猪气道上皮细胞（pAEC）响应猪和人源 IAV 诱导 I 型和 III 型 IFN 转录物的动力学和强度。我们观察到猪流感病毒 (SIV) 在 pAEC 中的复制效率比人类甲型流感/加利福尼亚/2009 (pH1N1 CA/09) 更有效。有趣的是，我们还发现这些病毒的 IFN-β、IFN-λ1 和 IFN-λ3 基因表达动力学存在显着差异。虽然感染猪 IAV A/Sw/Illinois/2008 (H1N1 IL/08) 的 pAEC 中 IFN 基因的诱导延迟了长达 12 小时，但人 pH1N1 CA/09 早在感染后 4 小时就迅速诱导了 IFN-β、IFN-λ1 和 IFN-λ3 基因表达。然而，注射后 24 小时时 IFN-β 和 IFN-λ3 诱导的强度并不明显。测试的病毒株之间没有显着差异。此外，我们发现与人 pH1N1 CA/09 相比，猪 H1N1 IL/08 对 dsRNA 诱导的抗病毒反应不太敏感。我们的数据表明，人类和猪 IAV 在猪细胞中诱导和响应 I 型和 III 型干扰素的能力不同。猪源性 IAV 可能通过破坏对病毒感染的先天抗病毒反应来适应猪宿主。
Interferons (IFNs) have been shown to inhibit influenza A virus (IAV) replication and play an essential role in controlling viral infection. Here we studied the kinetics and magnitude of induction of type I and type III IFN transcripts by primary porcine airway epithelial cells (pAECs) in response to swine and human origin IAV. We observed that swine influenza viruses (SIV) replicate more efficiently than the human pandemic influenza A/California/2009 (pH1N1 CA/09) in pAECs. Interestingly, we also found significant difference in kinetics of IFN-β, IFN-λ1 and IFN-λ3 gene expression by these viruses. While there was delay of up to 12 hours post infection (h p.i.) in induction of IFN genes in pAECs infected with swine IAV A/Sw/Illinois/2008 (H1N1 IL/08), human pH1N1 CA/09 rapidly induced IFN-β, IFN-λ1 and IFN-λ3 gene expression as early as 4 h p.i. However, the magnitude of IFN-β and IFN-λ3 induction at 24 h p.i. was not significantly different between the viral strains tested. Additionally, we found that swine H1N1 IL/08 was less sensitive to dsRNA induced antiviral response compared to human pH1N1 CA/09. Our data suggest that the human and swine IAVs differ in their ability to induce and respond to type I and type III interferons in swine cells. Swine origin IAV may have adapted to the pig host by subverting innate antiviral responses to viral infection.