Transgenic Rice Expressing Ictb and FBP/Sbpase Derived from Cyanobacteria Exhibits Enhanced Photosynthesis and Mesophyll Conductance to CO2.
Transgenic Rice Expressing Ictb and FBP/Sbpase Derived from Cyanobacteria Exhibits Enhanced Photosynthesis and Mesophyll Conductance to CO2.
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表达源自蓝藻的 Ictb 和 FBP/Sbpase 的转基因水稻表现出增强的光合作用和叶肉对 CO2 的传导性
DOI：
10.1371/journal.pone.0140928
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li YS
中科院分区：
文献类型：
--
作者：
Gong HY;Li Y;Fang G;Hu DH;Jin WB;Wang ZH;Li YS
为了找到一种提高水稻碳通量速率并进一步提高光合速率的方法，将分别构建的含有35S花椰菜花叶病毒启动子且来自蓝藻的两个二氧化碳转运和固定相关基因（Ictb和FBP/Sbpase）转入水稻。同时构建了分别含有Ictb、FBP/Sbpase以及这两个基因组合的三个同源转基因株系，并通过生理、生化和叶片解剖分析研究了这两个基因的功能效应。结果表明，与野生型相比，这三个株系的叶肉导度和净光合速率分别提高了约10.5 - 36.8%和13.5 - 34.6%，而叶片解剖结构没有任何变化。其他生理和生化参数在三个株系中也呈现相同的上升趋势，这表明FBP/SBPase对提高光合能力的作用优于ICTB，并且在ICTB + FBP/SBPase中存在累加效应。ICTB定位在细胞质中，而FBP/Sbpase成功转运至叶绿体。这两个基因可能整体上表现出协同作用以促进碳流和同化速率。讨论了多基因转化工程及其在提高水稻光合能力和产量方面的潜在应用。
To find a way to promote the rate of carbon flux and further improve the photosynthetic rate in rice, two CO2-transporting and fixing relevant genes, Ictb and FBP/Sbpase, which were derived from cyanobacteria with the 35SCaMV promotor in the respective constructs, were transformed into rice. Three homologous transgenic groups with Ictb, FBP/Sbpase and the two genes combined were constructed in parallel, and the functional effects of these two genes were investigated by physiological, biochemical and leaf anatomy analyses. The results indicated that the mesophyll conductance and net photosynthetic rate were higher at approximately 10.5–36.8% and 13.5–34.6%, respectively, in the three groups but without any changes in leaf anatomy structure compared with wild type. Other physiological and biochemical parameters increased with the same trend in the three groups, which showed that the effect of FBP/SBPase on improving photosynthetic capacity was better than that of ICTB and that there was an additive effect in ICTB+FBP/SBPase. ICTB localized in the cytoplasm, whereas FBP/SBPase was successfully transported to the chloroplast. The two genes might show a synergistic interaction to promote carbon flow and the assimilation rate as a whole. The multigene transformation engineering and its potential utility for improving the photosynthetic capacity and yield in rice were discussed.