Biosynthesis and function of simple amides in Xenorhabdus doucetiae
Biosynthesis and function of simple amides in Xenorhabdus doucetiae
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DOI：
10.1111/1462-2920.13919
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发表时间：
2017-11-01
影响因子：
5.1
通讯作者：
Bode, Helge B.
中科院分区：
文献类型：
--
作者：
Bode, Edna;He, Yue;Bode, Helge B.
昆虫病原线虫Steinernema diaprepesi的细菌共生体Xellobdus doucetiae产生几种不同的脂肪酸酰胺。它们的生物合成已经通过X中基因缺失和启动子交换的组合分析进行了研究。doucetiae中的候选基因的异源表达。杆菌虽然脱羧酶是形成所有观察到的苯乙基酰胺和色酰胺所必需的，但在黄杆菌素生物合成基因簇中编码的酰基转移酶XrdE负责形成短链酰基酰胺。此外，新的，长链和细胞毒性酰胺被确定在X。doucetiae感染的昆虫和X. doucetiae在Galleria Instant Broth（GIB）中生长。当测试所选酰胺的生物活性时，观察到短链酰基酰胺对来自色杆菌属和金黄杆菌属的两种不同群体感应系统的群体感应调节活性。
Xenorhabdus doucetiae, the bacterial symbiont of the entomopathogenic nematode Steinernema diaprepesi produces several different fatty acid amides. Their biosynthesis has been studied using a combination of analysis of gene deletions and promoter exchanges in X. doucetiae and heterologous expression of candidate genes in E. coli. While a decarboxylase is required for the formation of all observed phenylethylamides and tryptamides, the acyltransferase XrdE encoded in the xenorhabdin biosynthesis gene cluster is responsible for the formation of short chain acyl amides. Additionally, new, long-chain and cytotoxic acyl amides were identified in X. doucetiae infected insects and when X. doucetiae was grown in Galleria Instant Broth (GIB). When the bioactivity of selected amides was tested, a quorum sensing modulating activity was observed for the short chain acyl amides against the two different quorum sensing systems from Chromobacterium and Janthinobacterium.