Role of mitochondrial ribosome-dependent translation in germline formation in Drosophila embryos
Role of mitochondrial ribosome-dependent translation in germline formation in Drosophila embryos
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DOI：
10.1016/j.mod.2005.06.003
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发表时间：
2005-10-01
影响因子：
2.6
通讯作者：
Kobayashi, S
中科院分区：
文献类型：
--
作者：
Amikura, R;Sato, K;Kobayashi, S
在果蝇中，线粒体编码的核糖体rna （mtrrna）在极性颗粒（胚质的独特细胞器）上形成线粒体型核糖体。由于mtrRNA数量的减少导致胚胎不能产生种系祖细胞或极细胞，因此有人提出线粒体型核糖体的翻译是种系形成所必需的。在这里，我们报告了注射卡苏霉素（KA）和氯霉素（CH），原核型翻译抑制剂，破坏早期胚胎的极细胞形成。电镜观察显示，KA处理显著减少了极性颗粒上线粒体型核糖体的数量。相反，线粒体中的核糖体和线粒体活性不受KA和CH的影响。我们进一步发现，注射KA和CH会损害极细胞形成所需的无生殖细胞（Gcl）蛋白的产生。上述观察结果表明，线粒体型翻译是极细胞形成所必需的，Gcl可能是该翻译系统产生的蛋白质的候选者。2005爱思唯尔爱尔兰有限公司版权所有。
In Drosophila, mitochondrially encoded ribosomal RNAs (mtrRNAs) form mitochondrial-type ribosomes on the polar granules, distinctive organelles of the germ plasm. Since a reduction in the amount of mtrRNA results in the failure of embryos to produce germline progenitors, or pole cells, it has been proposed that translation by mitochondrial-type ribosomes is required for germline formation. Here, we report that injection of kasugamycin (KA) and chloramphenicol (CH), inhibitors for prokaryotic-type translation, disrupted pole cell formation in early embryos. The number of mitochondrial-type ribosomes on polar granules was significantly decreased by KA treatment, as shown by electron microscopy. In contrast, ribosomes in the mitochondria and mitochondrial activity were unaffected by KA and CH. We further found that injection of KA and CH impairs production of Germ cell-less (Gcl) protein, which is required for pole cell formation. The above observations suggest that mitochondrial-type translation is required for pole cell formation, and Gcl is a probable candidate for the protein produced by this translation system. (c) 2005 Elsevier Ireland Ltd. All rights reserved.