Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues
Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues
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DOI：
10.1152/ajpgi.00064.2011
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发表时间：
2011-07-01
影响因子：
4.5
通讯作者：
Yin, Dengping
中科院分区：
文献类型：
--
作者：
Burk, Raymond F.;Olson, Gary E.;Yin, Dengping
Burk RF，Olson GE，Winfrey VP，Hill RE，Yin D.由肾脏产生的谷胱甘肽过氧化物酶-3与胃肠道和其他组织中的基底膜结合。美国生理学胃肠和肝脏生理学杂志301：G32-G38，2011年。首次发表于2011年4月14日; doi：10.1152/ajpgi.00064.2011。谷胱甘肽过氧化物酶-3（Gpx 3）是主要在肾脏中合成的细胞外谷胱甘肽过氧化物酶，与肾皮质上皮细胞的基底膜结合。本研究采用RT-PCR和免疫细胞化学方法检测肾外Gpx 3表达和Gpx 3蛋白表达。Gpx 3在肾脏和附睾中的表达高于其他组织。Gpx 3与胃肠道上皮细胞的基底膜、连接生精小管与附睾的输出管、支气管和II型肺细胞结合。Ⅰ型肺泡上皮细胞基底膜未见表达。GPX 3也存在于附睾腔中。将Gpx 3（+/+）肾移植到Gpx 3（-/-）小鼠中导致Gpx 3与其在Gpx 3（+/+）小鼠中结合的相同基底膜结合，但不导致其在附睾腔中的存在。这些结果表明，来自血液的Gpx 3与特定上皮细胞的基底膜结合，并表明细胞修饰其基底膜以引起结合。它们进一步表明，在生物体中至少存在两个Gpx 3区室。在一个隔室中，肾脏通过血液向许多组织中的特定基底膜供应Gpx 3。在另一个隔室中，附睾将Gpx 3提供给其自身的管腔。肾脏和附睾以外的组织表达较低水平的Gpx 3，并可能向其他隔室提供Gpx 3。
Burk RF, Olson GE, Winfrey VP, Hill RE, Yin D. Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues. Am J Physiol Gastrointest Liver Physiol 301: G32-G38, 2011. First published April 14, 2011; doi:10.1152/ajpgi.00064.2011.-Glutathione peroxidase-3 (Gpx3), the extracellular glutathione peroxidase synthesized largely in the kidney, binds to basement membranes of renal cortical epithelial cells. The present study assessed extrarenal expression of Gpx3 using RT-PCR and presence of Gpx3 protein using immunocytochemistry. Gpx3 expression was higher in kidney and epididymis than in other tissues. Gpx3 bound to basement membranes of epithelial cells in the gastrointestinal tract, the efferent ducts connecting the seminiferous tubules with the epididymis, the bronchi, and type II pneumocytes. It was not detected on the basement membrane of type I pneumocytes. Gpx3 was also present in the lumen of the epididymis. Transplantation of Gpx3(+/+) kidneys into Gpx3(-/-) mice led to Gpx3 binding to the same basement membranes to which it bound in Gpx3(+/+) mice but not to its presence in the epididymal lumen. These results show that Gpx3 from the blood binds to basement membranes of specific epithelial cells and indicate that the cells modify their basement membranes to cause the binding. They further indicate that at least two Gpx3 compartments exist in the organism. In one compartment, kidney supplies Gpx3 through the blood to specific basement membranes in a number of tissues. In the other compartment, the epididymis provides Gpx3 to its own lumen. Tissues other than kidney and epididymis express Gpx3 at lower levels and may supply Gpx3 to other compartments.