Training and acute exercise modulates mitochondrial dynamics in football players' blood mononuclear cells
Training and acute exercise modulates mitochondrial dynamics in football players' blood mononuclear cells
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DOI：
10.1007/s00421-017-3684-z
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Pons, Antoni
中科院分区：
文献类型：
--
作者：
Busquets-Cortes, Carla;Capo, Xavier;Pons, Antoni
有规律的体育活动可诱导氧化应激，但也会引起抗氧化防御的适应，包括核因子κ B （nf - κ B）途径，该途径激活与抗氧化防御相关的靶基因，如解偶联蛋白（UCPs）和过氧化物酶体增殖体激活受体γ辅助激活因子1- α (PGC-1 α)介导的线粒体生物发生。该研究的目的是确定长期训练和急性运动对外周血单核细胞（PBMCs）氧化/抗氧化状态和线粒体生物发生基因表达的影响。方法12名职业足球运动员进行了为期8周的运动计划，其中包括每天2小时的足球训练。在训练季前后采集了血液样本。结果8周训练结束后，小鼠在静息状态下抗氧化蛋白水平和线粒体蛋白水平均显著升高。与运动前相比，急性运动后PGC1 α、UCP-2和mitofusin 2蛋白水平也有所升高。训练后，与运动前相比，急性运动后PGC1 α、细胞色素c氧化酶亚基IV和线粒体NADH脱氢酶亚基5信使RNA （mRNA）的表达显著增加；而在基础条件下，这些mRNA没有发生变化。急性运动后NF-kappa B激活和ROS生成显著增加。结论：训练增加了与线粒体生物发生相关的蛋白质水平，提高了训练有素的足球运动员pbmc中线粒体的抗氧化能力。急性运动可能通过nf - κ B激活和PGC1 α基因表达诱导线粒体生物发生。
Purpose Regular physical activity induces oxidative stress but also causes adaptations in antioxidant defences including the nuclear factor kappa B (NF-kappa B) pathway, which activates target genes related to antioxidant defences such as uncoupling proteins (UCPs), and mitochondrial biogenesis mediated by peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1 alpha). The aim of the study was to determine the effect of long-term training and acute exercise on oxidant/antioxidant status and the expression of mitochondrial biogenesis genes in peripheral blood mononuclear cells (PBMCs).Methods Twelve professional football players performed an 8-week exercise programme comprising a daily 2-h football training session. Blood samples were taken before and after the training season.Results The results reported a significant increase in antioxidant protein levels and in mitochondrial proteins in resting conditions after the 8-week training period. PGC1 alpha, UCP-2 and mitofusin 2 protein levels also increased after acute exercise compared to pre-exercise levels. After the training, the expression of PGC1 alpha, cytochrome c oxidase subunit IV and mitochondrial NADH dehydrogenase subunit 5 messenger RNA (mRNA) significantly augmented after the acute physical activity compared to pre-exercise levels; while no changes occurred in these mRNA in basal conditions. NF-kappa B activation and ROS production reported a significant increase after acute exercise.Conclusions Training increases the levels of proteins related to mitochondrial biogenesis and improves the antioxidant capabilities of mitochondria in PBMCs among well-trained football players. Acute exercise may act as an inducer of mitochondrial biogenesis through NF-kappa B activation and PGC1 alpha gene expression.