Microsatellite loci are not abundant in all arthropod genomes:: analyses in the hard tick, Ixodes scapularis and the yellow fever mosquito, Aedes aegypti
Microsatellite loci are not abundant in all arthropod genomes:: analyses in the hard tick, Ixodes scapularis and the yellow fever mosquito, Aedes aegypti
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DOI：
10.1046/j.1365-2583.2001.00260.x
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发表时间：
2001-06-01
影响因子：
2.6
通讯作者：
Black, WC
中科院分区：
文献类型：
--
作者：
Fagerberg, AJ;Fulton, RE;Black, WC
在蜱、肩胛伊蚊和埃及伊蚊中生成了微卫星富集的质粒文库。文库丰富了含有（AC）(n)、（AG）(n)、（ATG）(n)、（CAG）(n)、（TAG）(n)、（AAT）(n)、（CTGY）(n)或（GATA）(n)基序的基因组DNA。对两个物种中含有每个基序的克隆进行测序，用于PCR引物设计。在肩胛骨中，大多数引物扩增单个位点，等位基因微卫星重复数不同，并作为共显性标记分离。对比（AC）(n)、（TAG）(n)和（GATA）(n)从Ae. 3提取的微卫星位点。埃及伊蚊似乎是多基因家族的成员。设计用于扩增特定TAG位点的引物对扩增了许多独立分离的位点，其中一些不包含TAG微卫星。TAG位点上的等位基因作为显性标记分离，等位基因之间的长度变异证据有限。这些结果表明，微卫星位点在节肢动物基因组中并不普遍丰富，等位基因也并不总是作为共显性标记分离。
Plasmid libraries enriched for microsatellites were generated in the tick, Ixodes scapularis and in the mosquito Aedes aegypti. Libraries were enriched for genomic DNA containing (AC)(n), (AG)(n), (ATG)(n), (CAG)(n), (TAG)(n), (AAT)(n), (CTGY)(n) or (GATA)(n) motifs. Clones containing each motif were sequenced in both species for PCR primer design. In I. scapularis, most primers amplified a single locus and alleles varied in the number of microsatellite repeats and segregated as codominant markers. In contrast (AC)(n), (TAG)(n) and (GATA)(n) microsatellite loci extracted from Ae. aegypti appeared to be members of multigene families. A primer pair designed to amplify a particular TAG locus instead amplified many independently segregating loci, some of which did not contain TAG microsatellites. Alleles at the TAG loci segregated as dominant markers and there was limited evidence for length variation among alleles. These results suggest that microsatellite loci are not universally abundant in arthropod genomes nor do alleles always segregate as codominant markers.