Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells.
Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells.
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DOI：
10.1016/j.actbio.2010.02.038
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发表时间：
2010-09
影响因子：
9.7
通讯作者：
Fahsai Kantawong;Karl E. V. Burgess;Kamburapola Jayawardena;A. Hart;M. Riehle;R. Oreffo;M. Dalby;R. Burchmore
中科院分区：
文献类型：
--
作者：
Fahsai Kantawong;Karl E. V. Burgess;Kamburapola Jayawardena;A. Hart;M. Riehle;R. Oreffo;M. Dalby;R. Burchmore
人骨骼干细胞（STRO-1阳性/STRO-1+）以各种方式响应于不同的地形特征。在平坦表面上，这些细胞扩散并倾向于形成成纤维细胞样形态。在微槽表面上，富集的骨骼干细胞群体更喜欢沿着沿着槽伸展，这影响了它们的细胞结构和分化，这种现象称为接触引导。生长因子、激素和化学物质也可以刺激细胞分化。一种传统的中药，葛根，以前被观察到刺激骨形成。活性成分已被鉴定为具有雌激素样生物活性的甾酮。本研究结合了微槽拓扑结构和可生物降解聚合物聚己内酯（PCL）中的类β-内酯酮的影响。将人成骨细胞（STRO-1+）分别接种于平型PCL、槽型PCL和葛根粉浸渍槽型PCL上培养5周。考马斯亮蓝染色表明，在平坦PCL、沟槽PCL和用1 wt.%浸渍的沟槽PCL上，细胞生长和存活相似。或2重量%葛根粉浸渍有2重量%的带槽PCL观察到葛根粉对蛋白质表达有影响，如通过阳性骨钙素染色所观察到的。通过差异凝胶电泳分析蛋白质表达谱，以鉴定显示响应于这些不同环境的表达调节的蛋白质。总的来说，我们的研究结果表明，葛根粉具有相加作用，沿着微槽地形刺激，以促进影响细胞表型的STRO-1+蛋白质组的变化。
Human skeletal stem cells (STRO-1 positive/STRO-1+) respond to different topographical features in various ways. On a flat surface these cells spread and tend to develop a fibroblast-like morphology. On a microgrooved surface enriched skeletal stem cell populations prefer to stretch along the grooves, which affects their cellular structure and differentiation, a phenomenon known as contact guidance. Growth factors, hormones and chemicals can also stimulate cell differentiation. A traditional Chinese medicine, puerariae radix, has previously been observed to stimulate bone formation. The active ingredients have been identified as isoflavones with estrogen-like bioactivity. This study combined the effects of microgrooved topology and hormone-like isoflavones in the biodegradable polymer polycaprolactone (PCL). Human osteogenic cells (STRO-1+) were cultured on flat PCL, grooved PCL and puerariae powder-impregnated grooved PCL for 5weeks. Coomassie staining indicated that cell growth and survival was similar on flat PCL, grooved PCL and grooved PCL impregnated with 1 wt.% or 2 wt.% puerariae powder. Grooved PCL impregnated with 2 wt.% puerariae powder was observed to have an influence on protein expression, as observed by positive osteocalcin staining. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to these different environments. Overall, our results suggest that puerariae powder has an additive effect, along with microgrooved topographical stimulation, to promote changes in the STRO-1+proteome that affect cell phenotype.