DIFFERENCES IN B-CELL GROWTH PHENOTYPE REFLECT NOVEL PATTERNS OF EPSTEIN-BARR-VIRUS LATENT GENE-EXPRESSION IN BURKITT-LYMPHOMA CELLS
DIFFERENCES IN B-CELL GROWTH PHENOTYPE REFLECT NOVEL PATTERNS OF EPSTEIN-BARR-VIRUS LATENT GENE-EXPRESSION IN BURKITT-LYMPHOMA CELLS
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DOI：
10.1002/j.1460-2075.1987.tb02568.x
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发表时间：
1987-09-01
期刊：
影响因子：
11.4
通讯作者：
RICKINSON, AB
中科院分区：
文献类型：
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作者：
ROWE, M;ROWE, DT;RICKINSON, AB
最近建立的EB病毒（EBV）阳性伯基特淋巴瘤（BL）细胞系携带指示其恶性起源的染色体易位，已经监测了它们在体外向更"淋巴母细胞样"细胞表面表型和生长模式发展的程度，以及它们表达组成性存在于所有病毒转化的正常淋巴母细胞样细胞系中的三种EBV潜伏基因产物（LCL）。在连续传代中稳定保留原始肿瘤活检表型的BL细胞系对核抗原EBNA 1均呈阳性，但不表达可检测量的另外两种"转化"蛋白，EBNA 2和潜伏膜蛋白（LMP）。在BL活检组织的直接分析中也观察到这种新的EBV基因表达模式。所有三种病毒蛋白成为可检测的，但是，在BL细胞系已朝着更LCL样表型在体外。这项工作建立了B细胞表型和EB病毒潜伏基因表达的伴随模式之间的联系，并确定了一种新类型的EB病毒：细胞相互作用，这可能是独特的BL细胞。
Recently established Epstein-Barr virus (EBV)-positive Burkitt''s lymphoma (BL) cell lines, carrying chromosomal translocations indicative of their malignant origin, have been monitored for their degree of in vitro progression towards a more ''lymphoblastoid'' cell surface phenotype and growth pattern, and for their expression of three EBV latent gene products which are constitutively present in all virus-transformed normal lymphoblastoid cell lines (LCLs). BL cell lines which stably retained the original tumour biopsy phenotype on serial passage were all positive for the nuclear antigen EBNA 1 but did not express detectable amounts of two other ''transforming'' protein, EBNA 2 and the latent membrane protein (LMP). This novel pattern of EBV gene expression was also observed on direct analysis of BL biopsy tissue. All three viral proteins became detectable, however, in BL cell lines which had progressed towards a more LCL-like phenotype in vitro. This work establishes a link between B cell phenotype and the accompanying pattern of EBV latent gene expression, and identifies a novel type of EBV:cell interaction which may be unique to BL cells.