Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets
Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets
复制标题
油包水乳液液滴内表面微管的运动性
DOI：
10.1021/acs.langmuir.7b01550
复制
发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Kakugo Akira
中科院分区：
文献类型：
--
作者：
Tsuji Mikako;Rashedul Kabir Arif Md.;Ito Masaki;Inoue Daisuke;Kokado Kenta;Sada Kazuki;Kakugo Akira
油包水乳液体系近年来在各个领域引起了广泛的关注。然而，油包水乳液体系的功能化，这是扩大其在工业和研究中的应用所需要的，一直具有挑战性。我们现在展示了通过锚定靶蛋白质分子的油包水乳液系统的功能化。利用镍-氨三乙酸-组氨酸标签的特异性相互作用，将微管相关运动蛋白驱动蛋白1成功锚定在油包水型乳状液液滴的内表面。MT在驱动蛋白功能化的乳液液滴内表面上表现出滑动运动，这证实了油包水乳液系统的功能化的成功。这一结果将有利于探索生物分子马达系统在细胞膜上发生的细胞事件中的作用，也可能有助于扩大生物分子马达和油包水乳液系统在未来的纳米技术应用。
Water-in-oil emulsion systems have recently attracted much attention in various fields. However, functionalization of water-in-oil emulsion systems, which is required for expanding their applications in industries and research, has been challenging. We now demonstrate the functionalization of a water-in-oil emulsion system by anchoring a target protein molecule. A microtubule (MT)-associated motor protein kinesin-1 was successfully anchored to the inner surface of water-in-oil emulsion droplets by employing the specific interaction of nickel–nitrilotriacetic acid–histidine tag. The MTs exhibited a gliding motion on the kinesin-functionalized inner surface of the emulsion droplets, which confirmed the success of the functionalization of the water-in-oil emulsion system. This result would be beneficial in exploring the roles of biomolecular motor systems in the cellular events that take place at the cell membrane and might also contribute to expanding the nanotechnological applications of biomolecular motors and water-in-oil emulsion systems in the future.