Synthesis and release of sulfated glycoproteins by cultured glial cells.
Synthesis and release of sulfated glycoproteins by cultured glial cells.
复制标题
培养的神经胶质细胞合成和释放硫酸化糖蛋白。
DOI：
10.1016/0006-291x(85)90252-9
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Brunngraber,EG
中科院分区：
文献类型：
--
作者：
Bhat,NR;Brunngraber,EG
Both primary cultured glial cells and cloned (C-6) glioma cells have been shown to synthesize and release sulfated glycoproteins. It was found that N-linked tri-and tetra-antennary glycopeptides recovered from the glycoproteins contained most of the (35 S) sulfate label. C-6 glial cells showed a higher rate of oligosaccharide sulfation than the primary glial cultures. Both cell types exhibited a high rate of release of sulfated glycoproteins into the medium. The ratio of 35 S 3 H incorporated from (35 S) sulfate and (3 H) glucosamine in the released material was higher than that of the glycoproteins associated with the cell, indicating an enrichment of sulfated glycoproteins in the secreted materials. Monensin inhibited both the synthesis and the release of sulfated glycoproteins.
