THE FISSION YEAST MATING PHEROMONE P-FACTOR ITS MOLECULAR-STRUCTURE, GENE STRUCTURE, AND ABILITY TO INDUCE GENE-EXPRESSION AND G(1) ARREST IN THE MATING PARTNER
THE FISSION YEAST MATING PHEROMONE P-FACTOR ITS MOLECULAR-STRUCTURE, GENE STRUCTURE, AND ABILITY TO INDUCE GENE-EXPRESSION AND G(1) ARREST IN THE MATING PARTNER
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DOI：
10.1101/gad.8.3.328
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发表时间：
1994-02-01
影响因子：
10.5
通讯作者：
YAMAMOTO, M
中科院分区：
文献类型：
--
作者：
IMAI, Y;YAMAMOTO, M
Schizosaccharomyces pombe h（+）细胞分泌一种可扩散的交配信息素，称为p因子。在这里，我们展示了mag2基因，其缺陷赋予h（+）特异性不育，编码p因子的前体。我们从过表达map2的细胞中纯化p因子，并测定其氨基酸序列。p因子是一种由23个残基组成的肽，序列为thr - tyr - ala - asp - ph - leu - arg - ala - tyr - gln - ser - trp - asn - thr - ph - val - asn - pro - asp - arg - pro - asn - leu。该序列的合成肽具有与纯化的p因子相同的比活性和色谱图谱，表明p因子未被修饰。缺乏营养的h（-）细胞对p因子表现出形态反应。在这些细胞中，sxa2基因的转录被激活，该基因编码一种被认为可以降解p因子的蛋白酶。cyr1零突变体缺乏腺苷酸环化酶，细胞内cAMP含量很少，即使在营养物质存在的情况下也易受p因子的影响。cyr1和sx2突变的组合增强了这种易感性。在h(-) cyr1 sxa2细胞中，p因子不仅诱导了对交配的反应，而且还使细胞周期阻滞在G期。这证明了S. pombe交配信息素具有阻止细胞周期进程的能力，这在以前被通常的营养饥饿和随后的生长停滞的交配要求所掩盖。
Schizosaccharomyces pombe h(+) cells secrete a diffusible mating pheromone called P-factor. Here we show that the mag2 gene, a defect of which confers h(+)-specific sterility, encodes the precursor of P-factor. We purified P-factor from cells overexpressing map2 and determined its amino acid sequence. P-factor is a peptide of 23 residues, with the sequence Thr-Tyr-Ala-Asp-Phe-Leu-Arg-Ala-Tyr-Gln-Ser-Trp-Asn-Thr-Phe-Val-Asn-Pro-Asp-Arg-Pro-Asn-Leu. A synthetic peptide of this sequence gave the same specific activity and chromatographic profile as the purified P-factor, suggesting that P-factor is unmodified. h(-) cells starved for nutrition showed a morphological response to P-factor. Transcription of the sxa2 gene, which encodes a protease thought to degrade P-factor, was activated in these cells. The cyr1 null mutant, which lacks adenylyl cyclase and has little intracellular cAMP, was susceptible to P-factor even in the presence of nutrients. Combination of the cyr1 and sxa2 mutations enhanced this susceptibility. P-factor induced not only responses toward mating but also arrest of the cell cycle at the G, phase in h(-) cyr1 sxa2 cells. This proves that the S. pombe mating pheromone has the ability to arrest cell cycle progression, which has previously been obscured by the usual requirement for mating of nutritional starvation and subsequent growth arrest.