Measurement of total serum testosterone in adult men:: Comparison of current laboratory methods versus liquid chromatography-tandem mass spectrometry
Measurement of total serum testosterone in adult men:: Comparison of current laboratory methods versus liquid chromatography-tandem mass spectrometry
复制标题
DOI：
10.1210/jc.2003-031287
复制
发表时间：
2004-02-01
影响因子：
5.8
通讯作者：
Swerdloff, RS
中科院分区：
文献类型：
--
作者：
Wang, C;Catlin, DH;Swerdloff, RS
男性性腺功能减退症的诊断需要证明血清睾酮 (T) 水平较低。我们通过四种常用的自动免疫测定仪器（Roche Elecsys、Bayer Centaur、Ortho Vitros ECi 和 DPC Immulite 2000）和两种手动免疫测定方法（DPC-RIA（涂层管商业试剂盒）和 HUMC-RIA（研究实验室）检查了从 62 名性腺功能正常和 60 名性腺功能减退男性采集的谱系样本中的血清 T 水平。 测定）并将结果与液相色谱-串联质谱法（LC-MSMS）进行的测量进行比较。 Deming 的回归分析将每个测试结果与 LC-MSMS 进行比较，结果显示斜率在 0.881 和 1.217 之间。所有方法的组间相关系数均在 0.92 至 0.97 之间。与 LC-MSMS 测量的血清 T 浓度相比，在较宽的血清 T 水平范围内，DPC Immulite 结果偏向较低值（平均差，-90+/-9 ng/dl），而 Bayer Centaur 数据偏向较高值（平均差，+99+/-11 ng/dl）。血清 T 浓度低时（
The diagnosis of male hypogonadism requires the demonstration of a low serum testosterone (T) level. We examined serum T levels in pedigreed samples taken from 62 eugonadal and 60 hypogonadal males by four commonly used automated immunoassay instruments (Roche Elecsys, Bayer Centaur, Ortho Vitros ECi and DPC Immulite 2000) and two manual immunoassay methods (DPC-RIA, a coated tube commercial kit, and HUMC-RIA, a research laboratory assay) and compared results with measurements performed by liquid chromatography-tandem mass spectrometry (LC-MSMS). Deming's regression analyses comparing each of the test results with LC-MSMS showed slopes that were between 0.881 and 1.217. The interclass correlation coefficients were between 0.92 and 0.97 for all methods. Compared with the serum T concentrations measured by LC-MSMS, the DPC Immulite results were biased toward lower values (mean difference, -90+/-9 ng/dl) whereas the Bayer Centaur data were biased toward higher values (mean difference, +99+/-11 ng/dl) over a wide range of serum T levels. At low serum T concentrations (