Chemical cues from beetle larvae trigger proliferation and virulence of a plant pathogen

Chemical cues from beetle larvae trigger proliferation and virulence of a plant pathogen
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甲虫幼虫的化学信号触发植物病原体的增殖和毒力

DOI:
10.1101/2023.11.21.568124
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发表时间:
2023
期刊:
--
影响因子:
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通讯作者:
Cambon M
Cambon M
中科院分区:
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文献类型:
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作者:
Cambon M

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害虫和微生物病原体威胁农作物生产力和全球森林生物群落,昆虫-微生物相互作用可加剧植物衰退的严重程度。虽然细菌化学物质已被证明会影响昆虫的行为,但昆虫衍生的化学物质对植物病原体的影响几乎不为人所知。在这里,我们调查了甲虫幼虫-细菌的相互作用,在急性橡木衰退(AOD),橡木衰退疾病的特点是内部树皮坏死所造成的polymicrobial财团includingBrenneria goodwinii和幼虫画廊的Agrilus biguttatus。为了验证幼虫化学物质影响B.很好,我们提取了A。biguttatus幼虫的生长和基因表达的影响。体外培养良好。我们证明,幼虫化学品引起细菌生长速率和最终细胞密度增加,以及近800个基因的差异表达,包括III型分泌系统及其效应子,其他植物病原体中的主要毒力因子。相比之下,画廊木质组织提取物没有引起这样的影响。我们的工作突出了多王国相互作用在植物疾病中的重要性,并证明了昆虫衍生的化学激发子在促进植物病原体的毒力方面的新的作用模式,这可能对全球了解植物疾病很重要。
Pest insects and microbial pathogens threaten agricultural crop productivity and global forest biomes, and insect-microbe interactions can increase the severity of plant decline. Whilst bacterial chemicals have been shown to influence insect behaviour, the impact of insect-derived chemistries on phytopathogens is barely understood. Here, we investigated beetle larvae-bacteria interactions in acute oak decline (AOD), a decline disease of oak characterised by inner bark necrosis caused by a polymicrobial consortium includingBrenneria goodwiniiand larval galleries ofAgrilus biguttatus. To test the hypothesis that larval chemicals influence the activity ofB. goodwinii, we extractedA. biguttatuslarvae and tested the effect of extracts on the growth and gene expression ofB. goodwinii in vitro. We demonstrate that larval chemicals elicit increased bacterial growth rate and final cell density, as well as differential expression of almost 800 genes, including the Type III Secretion System and its effectors, major virulence factors in other plant pathogens. Contrastingly, gallery woody-tissue extracts induced no such effects. Our work highlights the importance of multi-kingdom interactions in plant disease and demonstrate a novel mode-of-action for insect-derived chemical elicitors in facilitating the virulence of phytopathogens, which may be important in understanding plant diseases globally.
DOI: 10.1073/pnas.97.16.8770
发表时间: 2000-08-01
影响因子: 11.1
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发表时间: 2011-06-01
影响因子: 1.7
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DOI: --
发表时间: 2016
期刊:
影响因子: --
作者:
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通讯作者: E. Jendek