Identification of genes differentially expressed in Mikania micrantha during Cuscuta campestris infection by suppression subtractive hybridization
Identification of genes differentially expressed in Mikania micrantha during Cuscuta campestris infection by suppression subtractive hybridization
复制标题
抑制消减杂交鉴定菟丝子感染期间薇甘菊差异表达基因
DOI：
10.1016/j.jplph.2009.02.002
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Peng, Shao-Lin
中科院分区：
文献类型：
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作者：
Li, Dong-Mei;Staehelin, Christian;Peng, Shao-Lin
从生物量积累、生理学和生态学方面研究了菟丝子对其寄主薇甘菊的影响。然而，人们对这种寄生植物与植物相互作用的分子事件知之甚少。在这项研究中，我们从野油菜感染诱导的微甘菊中鉴定出了新的基因。通过构建和表征来自微甘菊芽和茎的消减 cDNA 文库，研究了在渗透后早期被 C. Campestris 寄生时表达的基因。鉴定出 303 个可能上调的表达序列标签 (EST)，并将其分类为功能类别，例如“代谢”、“细胞防御和应激”、“转录因子”、“信号转导”、“运输”和“光合作用”。在受感染的微甘草的芽和茎中，诱导了与防御反应和细胞壁修饰相关的基因，证实了来自其他寄生植物与植物相互作用的类似数据。然而，发现受感染的枝条和茎中的基因表达谱是不同的。与受感染的芽相比，在受感染的茎中发现了更多响应生物和非生物胁迫因素诱导的基因。此外，数据库比较显示大量微甘草 EST 与未知功能的基因相匹配。通过定量实时 RT-PCR 对 21 个基因（来自不同功能类别）进行的表达分析显示，响应 C.campestris 感染，13 个转录物的水平显着增加。总之，本研究概述了被寄生的微甘草在渗透后早期阶段的基因。获得的数据构成了对寄生植物响应的宿主反应的分子理解的基础。皇冠版权所有 (C) 2009 由 Elsevier GmbH 出版。版权所有。
The influence of Cuscuta campestris on its host Mikania micrantha has been studied with respect to biomass accumulation, physiology and ecology. Molecular events of this parasitic plant-plant interaction are poorly understood, however. In this study, we identified novel genes from M. micrantha induced by C. campestris infection. Genes expressed upon parasitization by C. campestris at early post-penetration stages were investigated by construction and characterization of subtracted cDNA libraries from shoots and stems of M. micrantha. Three hundred and three presumably up-regulated expressed sequence tags (ESTs) were identified and classified in functional categories, such as "metabolism", "cell. defence and stress", "transcription factor", "signal transduction", "transportation" and "photosynthesis". In shoots and stems of infected M. micrantha, genes associated with defence responses and cell wall modifications were induced, confirming similar data from other parasitic plant-plant interactions. However, gene expression profiles in infected shoots and stems were found to be different. Compared to infected shoots, more genes induced in response to biotic and abiotic stress factors were identified in infected stems. Furthermore, database comparisons revealed a notable number of M. micrantha ESTs that matched genes with unknown function. Expression analysis by quantitative real-time RT-PCR of 21 genes (from different functional categories) showed significantly increased levels for 13 transcripts in response to C. campestris infection. In conclusion, this study provides an overview of genes from parasitized M. micrantha at early post-penetration stages. The acquired data form the basis for a molecular understanding of host reactions in response to parasitic plants. Crown Copyright (C) 2009 Published by Elsevier GmbH. All rights reserved.