Astrocyte and endothelial cell expression of ADAM 17 (TACE) in adult human CNS
Astrocyte and endothelial cell expression of ADAM 17 (TACE) in adult human CNS
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DOI：
10.1002/glia.1060
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发表时间：
2001-06-01
期刊：
影响因子：
6.2
通讯作者：
Woodroofe, MN
中科院分区：
文献类型：
--
作者：
Goddard, DR;Bunning, RAD;Woodroofe, MN
ADAM 17又称肿瘤血管紧张素转换酶，是肿瘤坏死因子-α、转化生长因子-α、L-选择素、p75、p55三种肿瘤坏死因子受体，以及白细胞介素1受体II等多种蛋白质的重要脱落酶。最近报道了成年小鼠和大鼠中枢神经系统中存在ADAM 17mRNA(Karkkainen等人)。Mol Cell Neurosci 15：547-560,2000)。然而，亚当17的细胞起源仍不清楚。在这项研究中，我们使用了一种抗ADAM 17的抗体来对其在成人中枢神经系统组织中的分布进行免疫组织化学研究。具有星形细胞和内皮细胞形态的细胞呈ADAM 17阳性。使用抗GFAP和von Willebrand因子的抗体的双重免疫荧光进一步证实了这一发现，这两种抗体分别标记星形胶质细胞和内皮细胞。这项研究表明，ADAM 17在正常脑组织中由星形胶质细胞和内皮细胞表达，可能在正常脑功能中发挥作用。(C)2001年Wiley-Liss，Inc.
ADAM 17, also known as TACE, is an important sheddase for a number of proteins, including tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-alpha (TGF-alpha), L-selectin, p75, and p55 TNF receptors, and interleukin-l receptor II (IL-1RII). The presence of ADAM 17 mRNA in adult mouse and rat CNS was recently reported (Karkkainen et al. Mol Cell Neurosci 15:547-560, 2000). However, the cellular origin of ADAM 17 remains unknown. In this study, we have used an anti-ADAM 17 antibody in an immunohistochemical study of its distribution in human adult CNS tissue. Cells with astrocytic and endothelial morphology were ADAM 17-positive. This finding was further confirmed using double immunofluorescence with antibodies against GFAP and von Willebrand factor, which label astrocytes and endothelial cells, respectively. This study demonstrates that ADAM 17 is expressed by astrocytes and endothelial cells in normal brain tissue and may have a role in normal brain function. (C) 2001 Wiley-Liss, Inc.