Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis
Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis
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DOI：
10.1093/rheumatology/keh250
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发表时间：
2004-09-01
期刊：
影响因子：
5.5
通讯作者：
Martini, A
中科院分区：
文献类型：
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作者：
Gattorno, M;Gregorio, A;Martini, A
目标。探讨慢性特发性关节炎滑膜骨桥蛋白（OPN）的表达及其与滑膜血管生成程度的关系。对45例活动性幼年特发性关节炎（JIA）患者进行了研究。所有患者在注射类固醇前均行SF抽吸。对22例JIA患者采集配对血浆样本。以15名年龄匹配的健康受试者的血浆作为对照。ELISA法检测血浆和SF中OPN和血管内皮生长因子（VEGF）的含量。在10例JIA患者的滑膜切除术中获得滑膜组织。采用抗opn、抗cd68、抗cd31、抗vegf和抗α (v) β(3)抗体，按标准技术进行免疫组化。SF组的OPN水平明显高于配对血浆样本(P
Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis.Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-alpha(v)beta(3) antibodies.Results. OPN levels were significantly higher in SF than in paired plasma samples (P