Antimicrobial blue light inactivation of Pseudomonas aeruginosa by photo-excitation of endogenous porphyrins: In vitro and in vivo studies.
Antimicrobial blue light inactivation of Pseudomonas aeruginosa by photo-excitation of endogenous porphyrins: In vitro and in vivo studies.
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DOI：
10.1002/lsm.22474
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发表时间：
2016-07
影响因子：
2.4
通讯作者：
Dai T
中科院分区：
文献类型：
--
作者：
Amin RM;Bhayana B;Hamblin MR;Dai T
铜绿假单胞菌是引起医院感染的最常见病原体之一，约占所有医院获得性感染的 10%。在本研究中，我们通过进行 10 个连续的亚致死蓝光灭活周期，研究了铜绿假单胞菌对抗菌蓝光的耐受性的潜在发展。采用高效液相色谱 (HPLC) 分析来鉴定铜绿假单胞菌细胞中的内源性卟啉。此外，我们使用铜绿假单胞菌生物发光菌株测试了抗菌蓝光在非致命性皮肤擦伤感染小鼠模型中的有效性。结果表明，经过 10 个亚致死灭活周期后，铜绿假单胞菌对抗菌蓝光没有产生耐受性。 HPLC 分析表明，铜绿假单胞菌能够产生内源性卟啉，特别是粪卟啉 III，其被认为是单独蓝光光动力效应的原因。在小鼠模型中，仅通过抗菌蓝光（48 J/cm2）即可根除铜绿假单胞菌感染，而无需添加任何光敏剂分子。总之，使用蓝光的内源性光敏作用作为皮肤感染的有效且安全的替代抗菌疗法应该受到广泛关注。
Pseudomonas aeruginosa is among the most common pathogens that cause nosocomial infections and is responsible for about 10% of all hospital-acquired infections. In the present study, we investigated the potential development of tolerance of P. aeruginosa to antimicrobial blue light by carrying 10 successive cycles of sublethal blue light inactivation. The high-performance liquid chromatographic (HPLC) analysis was performed to identify endogenous porphyrins in P. aeruginosa cells. In addition, we tested the effectiveness of antimicrobial blue light in a mouse model of nonlethal skin abrasion infection by using a bioluminescent strain of P. aeruginosa. The results demonstrated that no tolerance was developed to antimicrobial blue light in P. aeruginosa after 10 cycles of sub-lethal inactivation. HPLC analysis showed that P. aeruginosa is capable of producing endogenous porphyrins in particularly, coproporphyrin III, which are assumed to be responsible for the photodynamic effects of blue light alone. P. aeruginosa infection was eradicated by antimicrobial blue light alone (48 J/cm2) without any added photosensitizer molecules in the mouse model. In conclusion, endogenous photosensitization using blue light should gain considerable attention as an effective and safe alternative antimicrobial therapy for skin infections.