Comparative structural analysis of human and rat 65 kDa tumor-associated phosphoproteins.
Comparative structural analysis of human and rat 65 kDa tumor-associated phosphoproteins.
复制标题
人和大鼠 65 kDa 肿瘤相关磷蛋白的比较结构分析。
DOI：
10.1016/0020-711x(88)90318-7
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Hanausek,M
中科院分区：
文献类型：
--
作者：
Mirowski,M;Walaszek,Z;Sherman,U;Hanausek,M
1.从人和大鼠癌细胞培养基中分离出一种65 kDa的肿瘤相关蛋白（p65）。针对大鼠蛋白的抗体识别人类癌细胞系中的抗原相关蛋白。2. p65的氨基酸组成、N-末端和内部序列以及肽图和蛋白质印迹分析强烈地表明人和大鼠p65蛋白之间的高度同源性。3.同源性搜索表明，p65是不同源的，以前测序的蛋白质，但它可能与类固醇受体超家族的基因，特别是c-erb A基因的蛋白质。
1. A 65 kDa-tumor-associated protein (p65) was isolated from human and rat carcinoma cell culture media. Antibodies raised to the rat protein recognized an antigenically related protein in human cancer cell line. 2. Amino acid composition, N-terminal and internal sequence as well as peptide map and western blot analysis of the p65 strongly suggest a high degree of homology between the human and rat p65 proteins. 3. Homology searches indicated that p65 was not homologous to previously sequenced proteins, but that it may be related to proteins of the steroid receptor superfamily of genes, especially c-erb A gene.