The miR-532-3p/Chrdl1 axis regulates the proliferation and migration of amniotic fluid-derived mesenchymal stromal cells
The miR-532-3p/Chrdl1 axis regulates the proliferation and migration of amniotic fluid-derived mesenchymal stromal cells
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miR-532-3p/Chrdl1轴调节羊水源性间充质基质细胞的增殖和迁移
DOI：
10.1016/j.bbrc.2020.04.099
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发表时间：
2020-06-18
影响因子：
3.1
通讯作者：
Yuan, Zhengwei
中科院分区：
文献类型：
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作者：
Huang, Jieting;Wei, Xiaowei;Yuan, Zhengwei
背景：羊水源间充质基质细胞（AFMSCs）是一种很有前景的再生医学干细胞。然而，不同妊娠阶段分离的AFMSCs具有不同的生物学特性，体内和体外的治疗效果也不同。这些差异背后的机制尚未明确。方法：对AFMSC转录组进行生物信息学分析，鉴定出Chrdl1为差异表达基因之一。我们评估了Chrdl1过表达或敲低对AFMSCs增殖和迁移的影响。利用miRanda软件进行靶标预测，鉴定Chrdl1的上游microRNA。使用双荧光素酶报告基因测定法评估Chrdl1 mRNA与其上游microRNA之间的相互作用。结果：Chrdl1在妊娠早期的AFMSCs中表达水平较低。可抑制AFMSC的增殖和迁移。miR-532-3p通过靶向Chrdl1的3' UTR并下调其表达，促进AFMSC增殖和迁移。(c) 2020 Elsevier Inc.版权所有。
Background: Amniotic fluid-derived mesenchymal stromal cells (AFMSCs) are promising stem cells for regeneration medicine. However, AFMSCs isolated at different stages of pregnancy have different biological characteristics, and the therapeutic effects can differ in vivo and in vitro. The mechanisms underlying these differences have not been defined.Methods: Bioinformatics analysis of the AFMSC transcriptome identified Chrdl1 as one of the differentially expressed genes. We evaluated the effects of Chrdl1 overexpression or knockdown on the proliferation and migration of AFMSCs. Target prediction was performed using miRanda software to identify the upstream microRNA of Chrdl1. The interaction between Chrdl1 mRNA and its upstream microRNA was evaluated using a dual-luciferase reporter gene assay.Results: Chrdl1 was expressed at lower levels in AFMSCs derived from the early stages of pregnancy. It could suppress AFMSC proliferation and migration. miR-532-3p promoted AFMSC proliferation and migration by targeting the 3' UTR of Chrdl1 and downregulating its expression. (c) 2020 Elsevier Inc. All rights reserved.