ONTOGENY OF APOMORPHINE-INDUCED STEREOTYPY AND ITS D1 AND D2 RECEPTOR MEDIATION IN RATS DEPLETED OF DOPAMINE AS NEONATES
ONTOGENY OF APOMORPHINE-INDUCED STEREOTYPY AND ITS D1 AND D2 RECEPTOR MEDIATION IN RATS DEPLETED OF DOPAMINE AS NEONATES
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DOI：
10.1002/dev.420250703
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发表时间：
1992-11-01
影响因子：
2.2
通讯作者：
BRUNO, JP
中科院分区：
文献类型：
--
作者：
ABRAMS, DR;BRUNO, JP
用6-OHDA(100个/µg)或其载体于出生后第3天测定阿朴吗啡诱导的刻板行为的个体发生。在对照组和DA缺乏的动物中，早在第6天阿朴吗啡就产生了刻板的嗅闻和张口行为。从第6天到成年，DA缺乏的动物表现出对这些行为的超敏。通过测量特定的d1或d2受体拮抗剂在成人中阻断这些作用的能力来确定d1和d2受体激活对阿朴吗啡诱导的行为的相对贡献。用SCH 23390阻断D_1受体或用氯波必利阻断D_2受体均可抑制阿朴吗啡诱导的刻板反应。然而，在新生儿时期缺乏DA的成年大鼠对DA拮抗剂的敏感性低于对照组大鼠。这些结果表明，阿朴吗啡诱导的刻板印象早在出生后第6天就存在。新生时DA耗竭的大鼠继续表现出这些行为，即使在低于对照组动物所需的阿朴吗啡剂量下也是如此。最后，在两组大鼠中，D1和D2受体的共同激活对于阿朴吗啡诱导的刻板印象似乎是必要的。
The ontogeny of apomorphine-induced stereotypy in rats treated with 6-OHDA (100/mug) or its vehicle on postnatal Day 3 was determined on Days 6, 2 1, or 60-90. Stereotypic sniffing and mouthing behavior were produced by apomorphine in control and DA-depleted animals as early as Day 6. Animals depleted of DA as neonates exhibited supersensitivity to these behavioral effects from Day 6 into adulthood. The relative contributions of D1 and D2 receptor activation to apomorphine-induced behaviors were determined by measuring the ability of specific D1 or D2 antagonists to block these effects in adults. Blockade of D1 receptors with SCH 23390 or D2 receptors with clebopride suppressed apomorphine-induced stereotypy in both vehicle- and 6-OHDA-treated rats. However, adults depleted of DA as neonates were less sensitive to the DA antagonists than were control rats. These results demonstrate that apomorphine-induced stereotypy is present as early as postnatal Day 6. Rats depleted of DA as neonates continue to exhibit these behaviors, even at lower doses of apomorphine than were necessary in control animals. Finally, coactivation of D1 and D2 receptors appears necessary for apomorphine-induced stereotypy in both groups of rats.