The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.
The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.
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DOI：
10.3390/ijms22168931
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发表时间：
2021-08-19
影响因子：
5.6
通讯作者：
Pisetsky DS
中科院分区：
文献类型：
--
作者：
Spencer DM;Reyna AG;Pisetsky DS
DNA是一种高分子聚合物大分子，能够呈现多种主链构象。经典的B - DNA是右手双螺旋结构，而Z - DNA是具有锯齿状走向的左手螺旋结构。Z构象取决于碱基序列、碱基修饰和超螺旋，并且被认为是瞬时的。为了确定是否可以通过免疫化学方法检测到Z - DNA的存在，我们使用溴化多聚（dG - dC）作为Z - DNA的对照，通过酶联免疫吸附测定（ELISA）评估了单克隆和多克隆抗Z - DNA抗体与一组天然DNA抗原的结合情况。正如这些研究所示，在测试的天然DNA（藤黄微球菌、小牛胸腺、大肠杆菌、鲑鱼精子、λ噬菌体）中，藤黄微球菌（MC）DNA与两种抗Z - DNA制剂都显示出最高的结合率，大肠杆菌DNA与单克隆抗DNA制剂有结合。通过抑制结合试验证明了MC DNA中Z - DNA构象的特异性。一种识别形成Z - DNA倾向的算法表明，结核分枝杆菌的DNA能够形成Z - DNA，这一预测通过免疫测定得到了证实。总之，这些发现表明抗Z - DNA抗体可以作为检测不同物种来源的DNA中是否存在Z - DNA的探针，并且Z - DNA的含量在不同的DNA来源之间存在显著差异。
DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (Micrococcus luteus, calf thymus, Escherichia coli, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and E. coli DNA showed binding with the monoclonal anti-DNA preparation. The specificity for Z-DNA conformation in MC DNA was demonstrated by an inhibition binding assay. An algorithm to identify propensity to form Z-DNA indicated that DNA from Mycobacterium tuberculosis could form Z-DNA, a prediction confirmed by immunoassay. Together, these findings indicate that anti-Z-DNA antibodies can serve as probes for the presence of Z-DNA in DNA of various species origin and that the content of Z-DNA varies significantly among DNA sources.
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DOI：
10.1073/pnas.84.6.1560
发表时间：
1987-03-01
影响因子：
11.1
作者：
LANCILLOTTI, F;LOPEZ, MC;ALONSO, C
通讯作者：
ALONSO, C
影响因子：
2.9
作者：
MOLLER, A;NORDHEIM, A;RICH, A
通讯作者：
RICH, A
影响因子：
4.6
作者：
Beknazarov N;Jin S;Poptsova M
通讯作者：
Poptsova M
影响因子：
11.4
作者：
HO, PS;ELLISON, MJ;RICH, A
通讯作者：
RICH, A
DOI：
10.1073/pnas.78.3.1619
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BEHE, M;FELSENFELD, G
通讯作者：
FELSENFELD, G