Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum.
Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum.
复制标题
甘蓝型油菜 NPR1 样基因家族的全基因组鉴定以及 BnaNPR1 抗核盘菌的功能表征。
DOI：
10.1007/s00299-020-02525-z
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发表时间：
2020
影响因子：
6.2
通讯作者：
Tan Xiao-Li
中科院分区：
文献类型：
--
作者：
Wang Zheng;Ma Lu-Yue;Li Xiao;Zhao Feng-Yun;Sarwar Rehman;Cao Jun;Li Yu-Long;Ding Li-Na;Zhu Ke-Ming;Yang Yan-Hua;Tan Xiao-Li
关键信息BnaNPR 1样基因家族在B. BnaNPR 1的表达抑制显著降低了油菜对S.核盘菌（Sclerotiorum）加剧了活性氧（ROS）的积累，并改变了与SA和JA/ET信号相关的基因的表达，以响应该病原体。摘要病原相关基因的非表达子1（NPR 1）和相关的NPR 1样基因在调节植物防御中发挥重要作用。油菜（Brassica napusL.）是一种重要的油料作物;然而，人们对B知之甚少。napus（Bna）NPR 1-like基因家族。本研究共鉴定出19个BnaNPR 1样基因。napus基因组，然后根据它们各自在拟南芥中的最佳匹配（At）命名，从而确定了B.每个AtNPR 1样基因的napushomologs。对重要蛋白质结构域和功能基序的分析表明，这些同源物之间存在保守性和变异性。对这些BnaNPR 1样蛋白及其同源基因的系统发育分析显示，它们可分为6个不同的分支，表明它们的分类完全符合它们的系统发育关系。此外，B. napustranscriptomic数据显示，BnaNPR 1在油菜菌核病（Sclerotinia sclerotiorum）侵染后表达量显著下降，而BnaNPR 2/3/4/5/6在总体上没有表现出表达差异。此外，我们生成dB。通过对甘蓝型油菜BnaNPR 1-RNAi株系的分析，探讨BnaNPR 1基因表达下调对甘蓝型油菜抗S.菌核结果表明，BnaNPR 1-RNAi显著降低了该抗性。进一步的实验表明，BnaNPR 1-RNAi增强了植物与病原菌互作过程中ROS的产生，改变了植物的防御反应。这些结果表明S.菌核病可能利用BnaNPR 1来调节B.油菜的活性氧产生和SA防御反应等。
Key messageThe BnaNPR1-like gene family was identified inB. napus,and it was revealed that repression of BnaNPR1 significantly reduces resistance toS. sclerotiorum,intensifies ROS accumulation, and changes the expression of genes associated with SA and JA/ET signaling in response to this pathogen.AbstractTheNONEXPRESSOR OF PATHOGENESIS-RELATED GENES1(NPR1) and relatedNPR1-likegenes play an important role in regulating plant defense. Oilseed rape (Brassica napusL.) is an important oilseed crop; however, little is known about theB. napus(Bna)NPR1-likegene family. Here, a total of 19BnaNPR1-likegenes were identified in theB. napusgenome, and then named according to their respective best match inArabidopsis thaliana(At), which led to the determination ofB. napushomologs of everyAtNPR1-likegene. Analysis of important protein domains and functional motifs indicated the conservation and variation among these homologs. Phylogenetic analysis of these BnaNPR1-like proteins and theirArabidopsishomologs revealed six distinct sub-clades, consequently indicating that their name classification totally conformed to their phylogenetic relationships. Further,B. napustranscriptomic data showed that the expression of threeBnaNPR1swas significantly down-regulated in response to infection withSclerotinia sclerotiorum, the most important pathogen of this crop, whereasBnaNPR2/3/4/5/6sdid not show the expression differences in general. Further, we generatedB. napus BnaNPR1-RNAi lines to interpret the effect of the down-regulated expression ofBnaNPR1son resistance toS. sclerotiorum. The results showed thatBnaNPR1-RNAi significantly decreased this resistance. Further experiments revealed thatBnaNPR1-RNAi intensified ROS production and changed defense responses in the interaction of plants with this pathogen. These results indicated thatS. sclerotiorummight useBnaNPR1to regulate specific physiological processes ofB. napus, such as ROS production and SA defense response, for the infection.