Differential effects of overexpression of Rab5 and Rab22 on autophagy in PC12 cells with or without NGF.
Differential effects of overexpression of Rab5 and Rab22 on autophagy in PC12 cells with or without NGF.
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DOI：
10.1007/978-1-4939-2569-8_25
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Marlin MC;Li G
宏自噬通过靶向自噬小体，选择性地通过降解回收受损或不需要的蛋白质和细胞器。以下方法试图确定候选的Rab GTP酶，它可能在神经生长因子(NGF)饥饿期间调节PC12细胞的自噬。这种基于显微镜的分析是一种基于单细胞的定量检测自噬小体的存在，通过荧光标记的标记来响应RABS和突变体在存在或不存在NGF的情况下的过度表达。
Macroautophagy selectively recycles damaged or unneeded proteins and organelles by degradation via targeting to the autophagosome. The following method seeks to identify candidate Rab GTPases that likely modulate autophagy in PC12 cells during nerve growth factor (NGF) starvation. This microscopy-based assay is a single cell-based quantification of the presence of autophagosomes by fluorescently labeled markers in response to the overexpression of Rabs and mutants in the presence or absence of NGF.