A stable genomic source of P element transposase in Drosophila melanogaster.
A stable genomic source of P element transposase in Drosophila melanogaster.
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DOI：
10.1093/genetics/118.3.461
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发表时间：
1988-03
期刊：
影响因子：
3.3
通讯作者：
H. Robertson;C. Preston;R. Phillis;D. Johnson-Schlitz;Wendy K. Benz;W. Engels
中科院分区：
文献类型：
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作者：
H. Robertson;C. Preston;R. Phillis;D. Johnson-Schlitz;Wendy K. Benz;W. Engels
描述了果蝇中一种称为P[ry+delta2-3](99b)的单一P元素插入，它能以异常高的频率引起其他元素的动员，但本身却非常稳定。它的转座酶活性高于整个P菌株，但很少发生内部缺失、切除或转座。这种元素是由F.Laski，D.Rio和G.Rubin出于其他目的构建的，但我们发现它在涉及P元素的实验中很有用。我们证明，与含有大量非自治元素的染色体一起，它可以用于P元素的突变。在P元件介导的转化中，它还可以有效地替代“辅助”质粒，并可用于在基因组中移动转化的元件。
A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for "helper" plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.