A hybrid bioartificial liver composed of multiplated hepatocyte monolayers.
A hybrid bioartificial liver composed of multiplated hepatocyte monolayers.
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由多层肝细胞单层组成的混合生物人工肝。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
T. Kon
中科院分区：
文献类型：
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作者：
J. Uchino;T. Tsuburaya;F. Kumagai;T. Hase;T. Hamada;T. Komai;A. Funatsu;E. Hashimura;K. Nakamura;T. Kon
与悬浮细胞相比，单层培养的肝细胞具有良好的功能。制备了一种新型含肝细胞单层杂交人工肝，并对其功能进行了评价。从成年狗肝脏分离的肝细胞在胶原包被硼硅玻璃（10 X 20 X 0.04 cm）上培养。将200个承载肝细胞的玻璃板堆叠成长行程人工肝模块，灌注培养4周后肝细胞存活且功能良好；灌注第5天糖异生= 110 ng/微克DNA/min，尿素合成= 3.6 ng/微克DNA/min，白蛋白合成= 29微克/10(6)个细胞/天。维持两周。将新装置应用于无肝犬（第3组），并与未治疗犬（第1组）和血浆置换犬（第2组）进行比较。存活时间1组为21.3 +/- 5.6小时（N = 6）， 2组为27.8 +/- 4.0小时（N = 3）， 3组为55.0 +/- 10.3小时（N = 4）。最长存活时间为65小时。12 h后，1、2组血清氨升高至2000 μ g /dl以上，3组维持在400 μ g /dl以下。这种新型的混合系统可能是完整人工肝的先导设计。
Monolayer cultures of hepatocytes were shown to have good function when compared with suspended cells. The authors manufactured a new hybrid artificial liver containing hepatocyte monolayers and evaluated its function. Hepatocytes isolated from an adult dog liver were cultured on collagen coated borosilicated glass (10 X 20 X 0.04 cm). A long-stroke artificial liver module was constructed by stacking 200 glass plates bearing hepatocytes, which were viable and functioned well during 4 weeks in perfusion culture; glyconeogenesis = 110 ng/micrograms DNA/min, urea synthesis = 3.6 ng/micrograms DNA/min and albumin synthesis = 29 micrograms/10(6) cells/day at the 5th day of perfusion. The levels were maintained for 2 weeks. The new device was applied to anhepatic dogs (Group 3) and compared with untreated (Group 1) and plasma exchange dogs (Group 2). The survival times were 21.3 +/- 5.6 hours in Group 1 (N = 6), 27.8 +/- 4.0 hours in Group 2 (N = 3), and 55.0 +/- 10.3 hours in Group 3 (N = 4). The longest survival was 65 hours. Serum ammonia increased to over 2,000 micrograms/dl after 12 hours in Groups 1 and 2, but remained under 400 micrograms/dl in Group 3. This new type of hybrid system may be a pilot design for the complete artificial liver.