Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt's lymphoma.
Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt's lymphoma.
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伯基特淋巴瘤患者在清髓治疗后进行自体血源性造血干细胞移植。
DOI：
10.1182/blood.v67.2.529.bloodjournal672529
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发表时间：
1986
期刊：
影响因子：
20.3
通讯作者：
T. Fliedner
中科院分区：
文献类型：
--
作者：
M. Korbling;B. Dörken;A. Ho;A. Pezzutto;W. Hunstein;T. Fliedner
一名完全缓解的伯基特淋巴瘤患者接受了骨髓清除巩固治疗，采用超分次全身照射（1320 rad）和环磷酰胺（200 mg/kg），随后进行自体移植先前采集和冷冻保存的血源性造血干细胞。连续进行7次白细胞分裂，共产生55.2 × 10(9)个单核细胞（MNC），其中包括15.1 × 10(6)个CFU-GM或4.34 × 10(6)个cfu - gem。自体造血干细胞移植（american blood stem cell transplantation，简称abt）后，所有细胞系的重建都非常迅速，即9天后每微升可达到1000个白细胞，10天后每微升可达到500个粒细胞和50,000个血小板。B细胞在移植后35天左右达到正常值。CFU-GM首先出现在循环血液中，表现出巨大的超调。几天后，CFU-GM也出现在骨髓中。清髓治疗和脓肿移植后造血重建的动力学和模式提供了明确的证据，证明血源性造血干细胞能够完全恢复人的造血功能。一个可能的重建优势血液比骨髓来源的干细胞进行了讨论。
A patient with Burkitt's lymphoma in complete remission received myeloablative consolidation treatment with superfractionated total body irradation (1,320 rad) and cyclophosphamide (200 mg/kg) followed by autologous transplantation of previously harvested and cryopreserved blood-derived hemopoietic stem cells. Seven successive leukaphereses were performed to yield a total of 55.2 X 10(9) mononuclear cells (MNC) comprising 15.1 X 10(6) CFU-GM or 4.34 X 10(6) CFU-GEMM. Following autologous blood stem cell transplantation (ABSCT), reconstitution of all cell lines occurred very rapidly, ie, 1,000 leucocytes per microL were reached after nine days, 500 granulocytes and 50,000 platelets per microL after ten days. B cells reached normal values around day 35 post-transplantation. CFU-GM first appeared in the circulating blood exhibiting an enormous overshoot. Some days later CFU-GM also appeared in the marrow. The kinetics and pattern of hemopoietic reconstitution after myeloablative treatment and ABSCT provide clear evidence that blood-derived hemopoietic stem cells are capable of completely restoring hemopoietic function in man. A possible reconstitutive advantage of blood over marrow-derived stem cells is discussed.
人多能造血干细胞（CFU-GEMM）的体外研究。
影响因子：
20.3
作者：
Ash,RC;Detrick,DA;Zanjani,ED
通讯作者：
Zanjani,ED