Osmyb4 expression improves adaptive responses to drought and cold stress in transgenic apples
Osmyb4 expression improves adaptive responses to drought and cold stress in transgenic apples
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DOI：
10.1007/s00299-008-0587-9
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发表时间：
2008-10-01
影响因子：
6.2
通讯作者：
Mattana, Monica
中科院分区：
文献类型：
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作者：
Pasquali, Gemma;Biricolti, Stefano;Mattana, Monica
水稻冷诱导Osmyb4基因在转基因拟南芥（Arabidopsis thaliana）植物中的组成性表达提高了对寒冷和干旱胁迫的适应性反应，这很可能是由于几种胁迫诱导途径的组成性激活以及几种相容溶质（如葡萄糖、果糖、蔗糖、脯氨酸、甘氨酸、甜菜碱和一些芳香化合物）的积累。虽然Osmyb4基因似乎能够激活不同物种的应激反应途径，但我们之前报道过，它对应激耐受性的具体影响取决于转化的物种。在本工作中，我们报道了Osmyb4表达对改善苹果（Malus pumila Mill.）植物胁迫反应的影响。也就是说，我们发现Myb4转录因子的异位表达改善了对寒冷和干旱胁迫的生理生化适应，并改变了代谢物的积累。基于这些结果，利用Osmyb4作为一种工具来提高环境胁迫条件下木本多年生植物的生产力可能是有意义的。
Constitutive expression of the rice cold-inducible Osmyb4 gene in transgenic Arabidopsis (Arabidopsis thaliana) plants improves adaptive responses to cold and drought stress, most likely due to the constitutive activation of several stress-inducible pathways and to the accumulation of several compatible solutes (e.g., glucose, fructose, sucrose, proline, glycine betaine and some aromatic compounds). Although the Osmyb4 gene seems able to activate stress responsive pathways in different species, we previously reported that its specific effect on stress tolerance depends on the transformed species. In the present work, we report the effects of the Osmyb4 expression for improving the stress response in apple (Malus pumila Mill.) plants. Namely, we found that the ectopic expression of the Myb4 transcription factor improved physiological and biochemical adaptation to cold and drought stress and modified metabolite accumulation. Based on these results it may be of interest to use Osmyb4 as a tool for improving the productivity of woody perennials under environmental stress conditions.