Single-cell RNA sequencing identifies distinct mouse medial ganglionic eminence cell types.
Single-cell RNA sequencing identifies distinct mouse medial ganglionic eminence cell types.
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DOI：
10.1038/srep45656
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发表时间：
2017-03-31
影响因子：
4.6
通讯作者：
Modrusan Z
中科院分区：
文献类型：
--
作者：
Chen YJ;Friedman BA;Ha C;Durinck S;Liu J;Rubenstein JL;Seshagiri S;Modrusan Z
在成年小鼠皮质中发现了许多皮质中间神经元亚型，但产生其多样性的机制仍然难以捉摸。我们对小鼠胚胎内侧神经节隆起（MGE）（CIN的主要发源地）和从胚胎干细胞分化的MGE样细胞进行了单细胞RNA测序。两种不同的细胞类型被鉴定为增殖的神经祖细胞和未成熟的神经元，两者都包括亚群。虽然重建了MGE祖细胞的谱系发育，并且未成熟神经元被表征为GABA能，但未鉴定出可能对应于不同CIN前体的细胞。检测到一些非神经元细胞类型，包括小胶质细胞。体外MGE样细胞类似于真正的MGE细胞，但表达较低水平的Foxg1和Epha4。总之，我们的数据提供了详细的了解胚胎MGE发育程序，并建议如何CINs指定。
Many subtypes of cortical interneurons (CINs) are found in adult mouse cortices, but the mechanism generating their diversity remains elusive. We performed single-cell RNA sequencing on the mouse embryonic medial ganglionic eminence (MGE), the major birthplace for CINs, and on MGE-like cells differentiated from embryonic stem cells. Two distinct cell types were identified as proliferating neural progenitors and immature neurons, both of which comprised sub-populations. Although lineage development of MGE progenitors was reconstructed and immature neurons were characterized as GABAergic, cells that might correspond to precursors of different CINs were not identified. A few non-neuronal cell types were detected, including microglia. In vitro MGE-like cells resembled bona fide MGE cells but expressed lower levels of Foxg1 and Epha4. Together, our data provide detailed understanding of the embryonic MGE developmental program and suggest how CINs are specified.