Wolbachia infections of the butterfly Eurema mandarina interfere with embryonic development of the sawfly Athalia rosae
Wolbachia infections of the butterfly Eurema mandarina interfere with embryonic development of the sawfly Athalia rosae
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蝴蝶 Eurema mandarina 的沃尔巴克氏体感染干扰锯蝇 Athalia rosae 的胚胎发育
DOI：
10.1016/j.jip.2017.08.003
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发表时间：
2017
影响因子：
3.4
通讯作者：
Hatakeyama M
中科院分区：
文献类型：
--
作者：
Kageyama D;Wang C-H;Hatakeyama M
虽然母源传播的微生物如沃尔巴克氏体对不同昆虫物种的繁殖有多种影响，但其作用机制却知之甚少。在分类学上遥远的宿主物种之间人工转移沃尔巴克氏体可能会提供对沃尔巴克氏体诱导的宿主操纵的见解。在这里，我们进行了一个跨顺序转移feminizingWolbachiadrived从一只蝴蝶，Eurema courtina。Wolbachia在Eurema hecabecel细胞系中繁殖，称为NTU-YB，然后用于注射无Wolbachia的红腹叶蜂Athalia rosae的预蛹/蛹雌性。成年后出现的14只雌性动物在形态和行为上看起来都很健康，解剖时卵巢发育正常。然而，与对照组相比，7只注射沃尔巴克氏体的雌性所携带的333个卵中没有一个成功发育。同样地，四只注射了沃尔巴克氏体的雌虫与雄虫交配后，在寄主植物上产下的140个卵中没有一个显示出任何发育的迹象。在接种个体的全身样本中检测到沃尔巴克氏体感染，但在它们产下的卵中没有检测到。尽管卵细胞质中缺乏沃尔巴克氏体，但胚胎发育的中断可能代表了一种新的表型，涉及导致雌性不育的母体效应。
Although maternally transmitted microorganisms such asWolbachiaare well known to have a variety of effects on the reproduction of diverse insect species, little is known about the underlying mechanisms of actions. Artificial transfer ofWolbachiabetween taxonomically distant host species may provide insights intoWolbachia-induced manipulations of hosts. Here we performed a cross-order transfer of feminizingWolbachiaderived from a butterfly,Eurema mandarina. TheWolbachiawere propagated in theEurema hecabecell line, called NTU-YB, and then used to inject prepupal/pupal females of aWolbachia-free hymenopteran sawfly,Athalia rosae. The 14 females that emerged as adults looked morphologically and behaviorally healthy, and ovarian development appeared normal on dissection. However, in contrast to the control, none of the 333 eggs harbored by the sevenWolbachia-injected females developed successfully. Similarly, none of the 140 eggs laid on host plant by the fourWolbachia-injected females, which were mated with males, showed any signs of development.Wolbachiainfection was detected from whole-body samples of the inoculated individuals, but not from the eggs they produced. Disruption of embryonic development despite the absence ofWolbachiain the egg cytoplasm may represent a new phenotype involving maternal effects that result in female sterility.