Human MiR-4660 regulates the expression of alanine-glyoxylate aminotransferase and may be a biomarker for idiopathic oxalosis
Human MiR-4660 regulates the expression of alanine-glyoxylate aminotransferase and may be a biomarker for idiopathic oxalosis
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人 MiR-4660 调节丙氨酸乙醛酸转氨酶的表达，可能是特发性草酸中毒的生物标志物
DOI：
10.1007/s10157-019-01723-8
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发表时间：
2019-07-01
影响因子：
2.3
通讯作者：
Du, Dunfeng
中科院分区：
文献类型：
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作者：
Tu, Xin;Zhao, Yuanyuan;Du, Dunfeng
背景草酸合成功能障碍可导致草酸钙结石病和遗传性原发性高草酸尿症 (PH) 疾病。 I 型 PH (PH1) 是最严重的高草酸尿症之一，可导致尿石症、肾钙质沉着症和终末期肾病。在此，我们试图确定microRNA在调节AGXT中的作用，从而促进突变阴性特发性草酸中毒的发病机制。方法我们通过生物信息学寻找与AGXT结合的microRNA，并检测了草酸中毒患者血清样本、肝组织样本中最高命中（miR-4660）的表达，确定了microRNA之间的相关性和调控作用。 结果与健康对照相比，草酸中毒患者中 MiR-4660 表达下调（84.03 拷贝/μL vs 33.02 拷贝/μL，P
BackgroundDysfunction of oxalate synthesis can cause calcium oxalate stone disease and inherited primary hyperoxaluria (PH) disorders. PH type I (PH1) is one of the most severe hyperoxaluria disorders, which results in urolithiasis, nephrocalcinosis, and end-stage renal disease. Here, we sought to determine the role of microRNAs in regulating AGXT to contribute to the pathogenesis of mutation-negative idiopathic oxalosis.MethodsWe conducted bioinformatics to search for microRNAs binding to AGXT, and examined the expression of the highest hit (miR-4660) in serum samples of patients with oxalosis, liver tissue samples, and determined the correlation and regulation between the microRNA and AGXT in vitro.ResultsMiR-4660 expression was downregulated in patients with oxalosis compared with healthy controls (84.03 copies/mu L vs 33.02 copies/mu L, P