MORPHOLOGY AND PEROXIDASE CYTOCHEMISTRY OF MOUSE PROMONOCYTES, MONOCYTES, AND MACROPHAGES
MORPHOLOGY AND PEROXIDASE CYTOCHEMISTRY OF MOUSE PROMONOCYTES, MONOCYTES, AND MACROPHAGES
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DOI：
10.1084/jem.132.4.794
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发表时间：
1970-01-01
影响因子：
15.3
通讯作者：
FEDORKO, ME
中科院分区：
文献类型：
--
作者：
VANFURTH, R;HIRSCH, JG;FEDORKO, ME
小鼠前单核细胞已在骨髓细胞培养物中鉴定和研究。这些细胞的直径为14-20 µ，染色标本显示出一个大的、锯齿状或折叠的细胞核，以及嗜碱性的、细颗粒状的细胞质。相差显微镜下观察到的前单核细胞还显示了其他特征：核仁、小致密体、邻近核门的细胞质中的囊泡以及轻微的膜皱褶。前单核细胞的主要超微结构成分包括发达的高尔基体、少量的中心体颗粒和空泡、广泛的核糖体聚集体和细胞表面的指状突起。前单核细胞参与胞饮和吞噬作用，但它们在这些功能中的活性低于腹膜巨噬细胞的外周血单核细胞。前单核细胞过氧化物酶阳性，反应产物局限于颗粒，其中大部分颗粒位于电池的中心。血液或炎性腹膜渗出液中的单核细胞显示较少数量的过氧化物酶阳性颗粒，并且各种类型的成熟小鼠巨噬细胞为过氧化物酶阴性。
Mouse promonocytes have been identified and studied in cultures of bone marrow cells. These cells have a diameter of 14–20 µ, and in stained preparations reveal a large, indented or folded nucleus, and basophilic, finely granular cytoplasm. The living promonocyte viewed by phase contrast shows additional features: nucleoli, small dense bodies, and vesicles in the cytoplasm adjacent to the nuclear hilus, and slight membrane ruffling. Prominent ultrastructural components of promonocytes include a well developed Golgi apparatus, small numbers of centrosomal granules and vacuoles, extensive ribosomal aggregates, and finger-like projections of the cell surface.Promonocytes engage in pinocytosis and phagocytosis, but they are less active in these functions than are peripheral blood monocytes of peritoneal macrophages.Promonocytes are positive for peroxidase, the reaction product being localized to granules most of which are centrally situated in the cell. Monocytes in blood or in inflammatory peritoneal exudates display much smaller numbers of peroxidase-positive granules, and various types of mature mouse macrophages are peroxidase negative.