Examination of the causes of early pregnancy-associated thrombocytopenia in mice.
Examination of the causes of early pregnancy-associated thrombocytopenia in mice.
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检查小鼠早期妊娠相关血小板减少症的原因。
DOI：
10.1530/jrf.0.0730567
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
C. O'neill
中科院分区：
文献类型：
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作者：
C. O'neill
在小鼠中，与假孕相比，妊娠第2天的出血时间和全血凝血时间均无差异。将血小板浓度标准化为10（6）/μ L血浆导致妊娠动物血浆凝血时间显著缩短。这种减少不是由于凝血级联的内在或外在途径增加，而是由于血小板因子III活性增强，表明血小板活化和消耗增加。增加激活是不是由于胚胎的免疫识别，因为血小板减少症发生后，同系和同种异体交配的近交系小鼠，也孤雌激活后的卵子在原位。胚胎培养基注射到脾切除小鼠诱导一个显着的剂量依赖性血小板减少症。在注射后10 min内发生，并持续长达2 h。当给动物注射含有未受精卵的培养液时，血小板计数没有减少。早孕相关性血小板减少症是由受精卵产生的血小板活化因子引起的。胚胎培养液诱导血小板减少的剂量反应曲线与血小板活化因子1-O-烷基-2-乙酰基-sn-甘油（3）磷酸胆碱的剂量反应曲线平行。
In mice, neither the bleeding time nor the clotting time of whole blood was different on Day 2 of pregnancy compared with pseudopregnancy. Standardization of the platelet concentration to 10(6)/microliters plasma resulted in a significant reduction in the clotting time of plasma from pregnant animals. This reduction was not due to an increase in the intrinsic or extrinsic pathways of the coagulation cascade but to enhanced platelet factor III activity, indicating increased platelet activation and consumption. Increased activation was not due to immunological recognition of the embryo because thrombocytopenia occurred after syngeneic and allogeneic matings of inbred strains of mice and also after parthenogenetic activation of ova in situ. Injection of embryo culture medium into splenectomized mice induced a significant dose-dependent thrombocytopenia. It occurred within 10 min after injection and persisted for up to 2 h. There was no reduction in platelet count when animals were injected with culture media in which unfertilized ova had been incubated. Early pregnancy-associated thrombocytopenia was caused by the production of platelet-activating factors by the fertilized eggs. The induction of thrombocytopenia by embryo culture media displayed a dose-response curve that was parallel to that of the platelet-activating factor, 1-0-alkyl-2-acetyl-sn-glycero(3)phosphocholine.