Building the central complex in Drosophila: the generation and development of distinct neural subsets.
Building the central complex in Drosophila: the generation and development of distinct neural subsets.
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构建果蝇的中央复合体：不同神经亚群的产生和发展。
DOI：
10.1002/cne.22285
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Young JM
中科院分区：
文献类型：
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作者：
Young JM
昆虫大脑最显著的特征之一是独特的中线神经纤维，即中央复合体(Cx)。我们用两种细胞黏附分子dN-钙粘蛋白和棘球蛋白的免疫组织化学染色，以及一组7个增强子捕捉系，研究了果蝇嘴巴中四种Cx结构和几组Cx神经元的发育。我们的结果表明，Cx在三龄幼虫中首次可识别，并在变态的前48小时内详细说明。以未成熟形态出现的第一批可识别的结构是原脑桥和扇形小体，它们存在于3龄幼虫的大脑中，其次是结节(从P12h开始)，最后是椭圆形小体(从P24开始)。我们观察到，神经元逐渐增加到发育中的CX结构，小视野神经元的集合先于大视野神经元投射到CX。小视野神经元首先投射到发育中的扇形身体，然后树枝或延伸到其他结构。我们发现有证据表明，小视野神经元存在于16个一组的神经元中，它们来自皮质中八个常见的核周簇，这表明它们有共同的起源。我们还发现了一组新的脑桥神经元，它们连接着扇形身体的对侧节段。J.Comp.神经。518：1525-1541,2010。©2009威利-利斯，Inc.
The distinctive midline neuropil, the central complex (CX), is one of the most prominent features of the insect brain. We investigated the development of the four CX structures and several sets of CX neurons in theDrosophilabrain using immunostaining for two cell adhesion molecules, DN‐cadherin and Echinoid, and a set of seven enhancer trap lines. Our results showed that the CX is first identifiable in the third instar larva and that it elaborates over the first 48 hours of metamorphosis. The first identifiable structures to appear in their immature form are the protocerebral bridge and fan‐shaped body, which are present in the brain of the third instar larva, followed by the noduli (from P12h), and finally the ellipsoid body (from P24h). We observed that neurons are added incrementally to the developing CX structure, with sets of small‐field neurons projecting to the CX prior to the large‐field neurons. The small‐field neurons first project to the developing fan‐shaped body, before arborizing or extending to the other structures. We found evidence to suggest that small‐field neurons exist in sets of 16 and that they originate from eight common clusters of perikarya in the cortex, suggesting a common origin. We also identified a novel set of pontine neurons that connect contralateral segments in the fan‐shaped body. J. Comp. Neurol. 518:1525–1541, 2010. © 2009 Wiley‐Liss, Inc.